anti-Human Chromogranin A antibody for Western Blotting

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Epitope
C-Term
17
15
13
12
11
8
8
6
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
1004
316
258
206
167
123
11
10
5
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
685
320
22
7
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
683
633
460
404
306
212
211
196
168
102
16
13
13
8
7
3
2
1
1
Options
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Catalog No. ABIN2777071
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0806582 ABIN2777070 WB Rabbit N-Term Log in to see Polyclonal 1
3.0806582 ABIN2705879 WB Rabbit Center Log in to see Polyclonal 0
3.0806582 ABIN3042749 IHC (p) WB Rabbit IgG AA 321-457 Log in to see Polyclonal 0
3.0806582 ABIN4880257 ELISA FACS ICC WB Mouse IgG1 Log in to see 1A6B6 0
3.0806582 ABIN1724755 ICC FACS ELISA WB Mouse IgG1 AA 87-252 Log in to see 1A6B6 2
3.0806582 ABIN1845524 ELISA FACS IF/ICC WB Mouse IgG1 AA 87-252 Log in to see 0
3.0806582 ABIN1845525 ELISA WB Mouse IgG1 AA 87-252 Log in to see 0
3.0806582 ABIN782319 EIA IHC (p) WB Mouse IgG1 AA 19-131 Log in to see 3A5 0
3.0806582 ABIN4880644 ELISA WB Mouse IgG1 Log in to see 1A6B6 0
3.0806582 ABIN1724756 ELISA WB Mouse IgG1 AA 87-252 Log in to see 1A6B6 2
3.0806582 ABIN3024531 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CHGA-777 0
3.0806582 ABIN1174343 ICC IHC WB Rabbit IgG AA 15-148 Log in to see Polyclonal 0
3.0806582 ABIN2465199 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.0806582 ABIN4298554 IF IHC WB Mouse IgG1 Log in to see 2E12 0
3.0806582 ABIN4958732 IHC IHC (p) WB Mouse IgG1 Log in to see CL0166 0
3.0806582 ABIN2681794 WB Mouse IgG2a N-Term Log in to see 03 0
3.0806582 ABIN5945883 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see JM103-7 0
3.0806582 ABIN2681803 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0806582 ABIN2681800 WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0806582 ABIN5963146 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Chromogranin A (CHGA) Antibodies
Epitope C-Term
(17), (15), (13), (12), (11), (8), (8), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(1004), (316), (258), (206), (167), (123), (11), (10), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(685), (320), (22), (7), (1), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(683), (633), (460), (404), (306), (212), (211), (196), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN974912
Sequence DSLEAGLPLQ VRGYPEEKKE EEGSANRRPE DQELESLSAI EAELEKVAHQ
Predicted Reactivity Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CHGA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CHGA

Target Details

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Antigen
Alternative Name CHGA (CHGA Antibody Abstract)
Background CHGA is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. Its gene?s product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
Alias Symbols: CGA
Protein Interaction Partner: PARK2, UBC, CDC37, PFDN1, NEK2, SCG3, PLG,
Protein Size: 457
Molecular Weight 51 kDa
Gene ID 1113
NCBI Accession NM_001275, NP_001266
UniProt P10645
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 457 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Leibovitch, Pinthus, Sella, Ramon: "[Plasma chromogranin-A (CgA)--a potential marker for diagnosis, monitoring and management of prostate cancer patients]." in: Harefuah, Vol. 145, Issue 1, pp. 25-9, 78, 2006

Images

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Supplier Images
Western Blotting (WB) image for anti-Chromogranin A (CHGA) (C-Term) antibody (ABIN2777071) WB Suggested Anti-CHGA Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat ce...