anti-Human Chromogranin A antibody for Western Blotting

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Epitope
C-Term
30
16
15
14
12
8
8
6
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
706
201
187
130
119
51
11
11
6
6
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
471
233
22
7
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
26
22
18
18
17
17
16
16
16
16
16
16
16
16
16
15
13
8
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
502
395
386
326
219
216
169
117
109
31
13
13
7
4
3
2
2
1
1
1
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Catalog No. ABIN2777071
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2307212 ABIN2777070 WB Rabbit N-Term Log in to see Polyclonal 1
3.2307212 ABIN2705879 WB Rabbit Center Log in to see Polyclonal 0
3.2307212 ABIN3042749 IHC (p) WB Rabbit IgG AA 321-457 Log in to see Polyclonal 0
3.2307212 ABIN1724755 ICC FACS ELISA WB Mouse IgG1 AA 87-252 Log in to see 1A6B6 2
3.2307212 ABIN449786 IHC (p) IHC SDS ELISA WB Mouse IgG1 kappa AA 19-131 Log in to see 3A5 0
3.2307212 ABIN782319 EIA IHC (p) WB Mouse IgG1 AA 19-131 Log in to see 3A5 0
3.2307212 ABIN251488 ELISA WB Goat Log in to see Polyclonal 1
3.2307212 ABIN1724756 ELISA WB Mouse IgG1 AA 87-252 Log in to see 1A6B6 2
3.2307212 ABIN3024531 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CHGA-777 0
3.2307212 ABIN1174343 ICC IHC WB Rabbit IgG AA 15-148 Log in to see Polyclonal 0
3.2307212 ABIN4298554 IF IHC WB Mouse IgG1 Log in to see 2E12 0
3.2307212 ABIN2465199 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.2307212 ABIN2681794 WB Mouse IgG2a N-Term Log in to see 03 0
3.2307212 ABIN2681800 WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.2307212 ABIN2681803 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.2307212 ABIN2462501 ELISA WB Rabbit Log in to see Polyclonal 0
3.2307212 ABIN2462502 ELISA WB Rabbit Log in to see Polyclonal 0
3.2307212 ABIN2681796 WB Rabbit IgG N-Term Log in to see 026 0
3.2307212 ABIN2681804 WB Rabbit IgG N-Term Log in to see 039 0
3.2307212 ABIN1845524 ELISA FACS IF/ICC WB Mouse IgG1 AA 87-252 Log in to see 0

General

Antigen Chromogranin A (CHGA) Antibodies
Epitope C-Term
(30), (16), (15), (14), (12), (8), (8), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(706), (201), (187), (130), (119), (51), (11), (11), (6), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(471), (233), (22), (7), (1), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(26), (22), (18), (18), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (16), (15), (13), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(502), (395), (386), (326), (219), (216), (169), (117), (109), (31), (13), (13), (7), (4), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN974912
Sequence DSLEAGLPLQ VRGYPEEKKE EEGSANRRPE DQELESLSAI EAELEKVAHQ
Predicted Reactivity Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CHGA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CHGA

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHGA (CHGA Antibody Abstract)
Background CHGA is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. Its gene?s product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
Alias Symbols: CGA
Protein Interaction Partner: PARK2, UBC, CDC37, PFDN1, NEK2, SCG3, PLG,
Protein Size: 457
Molecular Weight 51 kDa
Gene ID 1113
NCBI Accession NM_001275, NP_001266
UniProt P10645
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 457 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Leibovitch, Pinthus, Sella, Ramon: "[Plasma chromogranin-A (CgA)--a potential marker for diagnosis, monitoring and management of prostate cancer patients]." in: Harefuah, Vol. 145, Issue 1, pp. 25-9, 78, 2006

Images

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Supplier Images
Western Blotting (WB) image for anti-Chromogranin A (CHGA) (C-Term) antibody (ABIN2777071) WB Suggested Anti-CHGA Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Western Blotting (WB) image for anti-Chromogranin A (CHGA) (C-Term) antibody (ABIN2777071) WB Suggested Anti-CHGA
Antibody Titration: 0.2-1 µg/mL
Positive Control...