anti-Rat (Rattus) Chromogranin A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human, Rat (Rattus)
991
311
254
203
167
117
12
11
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
678
316
21
7
1
1
Clonality
Monoclonal
Conjugate
Un-conjugated
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
673
623
451
397
302
212
204
191
161
95
13
13
9
8
7
3
2
1
1
Supplier
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Catalog No. ABIN3024534
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13 ABIN3024525 FACS IF IHC (p) Mouse IgG Log in to see CGA-413-CHGA-777-CHGA-798 0
12.972993 ABIN266232 ICC IF IHC (p) IHC Rabbit Log in to see Polyclonal 10
11.497463 ABIN214090 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.5 ABIN669846 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 420-457 Log in to see Polyclonal 5
7 ABIN3024549 FACS IF IHC (p) Mouse IgG1, kappa Log in to see LK2H10 0
4 ABIN385635 IHC IHC (p) WB Mouse IgG1, kappa Log in to see LK2H10 0
4 ABIN800424 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
4 ABIN3024547 FACS IF IHC (p) Mouse IgG1, kappa Log in to see SPM339 0
4 ABIN2472988 IHC (p) ELISA Sheep IgG Log in to see Polyclonal 0
4 ABIN187905 IHC (p) Rabbit Log in to see Polyclonal 0
2.472993 ABIN5945883 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see JM103-7 0
1 ABIN669855 IHC (p) WB HRP Rabbit IgG AA 420-457 Log in to see Polyclonal 0
1 ABIN669848 IHC (p) WB Biotin Rabbit IgG AA 420-457 Log in to see Polyclonal 0
1 ABIN470741 IF IHC IHC (fro) IHC (p) WB Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN2892579 IHC IHC (p) WB Rabbit Gly360 Log in to see Polyclonal 0
1 ABIN609406 ELISA IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2739940 ELISA FACS IF IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN112218 IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see LK2H10 1
1 ABIN6251925 FACS IF IHC (p) Mouse IgG1, kappa Log in to see CHGA-798 0
1 ABIN5646861 FACS IF IHC (p) Mouse IgG1 Log in to see LK2H10-PHE5-CGA414 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human, Rat (Rattus)
(991), (311), (254), (203), (167), (117), (12), (11), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(678), (316), (21), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(673), (623), (451), (397), (302), (212), (204), (191), (161), (95), (13), (13), (9), (8), (7), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Chromogranin A antibody.
Clone CHGA-798
Isotype IgG1, kappa

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal dilution of the Chromogranin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

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Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A ant...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-fixed, paraffin-embedded human parathyroid stained with Chromogranin A antib...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin human pancreas stained with Chromogranin A antibody (CHGA/798)
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin human SC lung carcinoma stained with Chromogranin A antibody (CHGA/...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin rat pancreas stained with Chromogranin A antibody (CHGA/798)