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|Antigen||Follistatin (FST) Antibodies|
|Conjugate||This Follistatin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-Follistatin AntibodyTarget Details Follistatin Application Details Handling Images
|Specificity||This antibody detetcs Follistatin.|
|Immunogen||Highly pure (> 98 %) recombinant human Follistatin|
|Plasmids, Primers & others|
Target Details FollistatinProduct Details anti-Follistatin Antibody Application Details Handling Images back to top
|Alternative Name||Follistatin (FST Antibody Abstract)|
|Background||Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing.Synonyms: Activin-binding protein, FS, FST|
|Pathways||Negative Regulation of Hormone Secretion|
Application DetailsProduct Details anti-Follistatin Antibody Target Details Follistatin Handling Images back to top
ELISA: Direct: To detect hFollistatin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanFollistatin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Follistatin Antibody Target Details Follistatin Application Details Images back to top
|Reconstitution||Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.|
|Buffer||PBS, pH 7.2|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.|
|Expiry Date||6 months|