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|Antigen||Follistatin (FST) Antibodies|
|Conjugate||This Follistatin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-Follistatin AntibodyTarget Details Follistatin Application Details Handling Images
E.coli derived recombinant Human Follistatin.
Type of Immunogen: Cell extract
Target Details FollistatinProduct Details anti-Follistatin Antibody Application Details Handling Images back to top
|Alternative Name||FST / Follistatin (FST Antibody Abstract)|
Name/Gene ID: FST
Synonyms: FST, Activin-binding protein, Follistatin isoform FST317, Follistatin, Fs
|Pathways||Negative Regulation of Hormone Secretion|
Application DetailsProduct Details anti-Follistatin Antibody Target Details Follistatin Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC-P (5 μg/mL), Neut (8 - 12 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7434 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100 ng/mL), a concentration of 8-12 μg/mL of this antibody is required. ELISA: To detect Human Follistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (LS-C104465) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Follistatin. Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Follistatin Antibody Target Details Follistatin Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-Follistatin Antibody Target Details Follistatin Application Details Handling back to top