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|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Conjugate||This EGF antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images
E.coli derived recombinant Human EGF.
Type of Immunogen: Cell extract
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top
|Alternative Name||EGF (EGF Antibody Abstract)|
Name/Gene ID: EGF
Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.25 - 0.35 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL-0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top