anti-Human Reticulon 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Reticulon 4 Antibody (supplied by: Log in to see )

Antigen
Reticulon 4 (RTN4) Antibodies
  • ASY
  • NI220/250
  • NOGO
  • NOGO-A
  • NOGOC
  • NSP
  • NSP-CL
  • Nbla00271
  • Nbla10545
  • Nogo-B
  • Nogo-C
  • RTN-X
  • RTN4-A
  • RTN4-B1
  • RTN4-B2
  • RTN4-C
  • RTN4-Cw
  • DKFZp459C0314
  • 1110020G17Rik
  • AA407876
  • AA409940
  • AA960376
  • C130026I10Rik
  • NgA
  • Nogo-A
  • mKIAA0886
  • mKIAA4153
  • NI-250
  • Nogo
  • Vp20
  • r
  • rat N
  • rat NogoA
  • reticulon-4
  • nogo
  • rtn4
  • wu:fb17a02
  • wu:fb22f12
  • wu:fd61a07
  • zgc:92163
  • reticulon 4
  • reticulon 4a
  • RTN4
  • rtn4
  • Rtn4
  • rtn4a
Reactivity
Human, Mouse (Murine), Rat (Rattus)
135
106
103
10
8
8
8
8
5
5
4
3
1
1
1
Host
Rabbit
140
11
4
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
6
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
119
62
54
36
26
11
4
4
3
2
2
1
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Catalog No. ABIN4340280
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.280954 ABIN2140414 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
13.280954 ABIN1689916 ICC IHC (p) WB Rabbit Log in to see Polyclonal 0
11.5841255 ABIN343914 ELISA IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
11.5841255 ABIN343915 ELISA IHC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
11.5841255 ABIN615504 IHC (p) Goat Log in to see Polyclonal 0
11.5 ABIN541599 IHC (p) WB Rabbit Isoform 1 Log in to see Polyclonal 3
11.5 ABIN541598 IHC (p) WB Rabbit Isoform 1 Log in to see Polyclonal 3
11.458544 ABIN4340304 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal 0
10 ABIN574094 IHC IHC (p) Goat AA 501-515 Log in to see Polyclonal 0
10 ABIN3032463 IHC (p) WB Rabbit IgG AA 1170-1192 Log in to see Polyclonal 0
8.5 ABIN2475901 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 4
7 ABIN959415 IHC IHC (p) WB Rabbit AA 14-30 Log in to see Polyclonal 0
7 ABIN372912 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
7 ABIN372913 EIA IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
7 ABIN1738971 IHC (p) Goat Internal Region Log in to see Polyclonal 0
4 ABIN1108866 IF IHC (p) IP WB Rabbit IgG C-Term, Isoform 1 Log in to see Polyclonal 0
4 ABIN1492925 ICC IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
4 ABIN725480 IF (p) IHC (p) WB Rabbit IgG AA 350-400 Log in to see Polyclonal 0
4 ABIN5958754 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1738973 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen Reticulon 4 (RTN4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(135), (106), (103), (10), (8), (8), (8), (8), (5), (5), (4), (3), (1), (1), (1)
Host Rabbit
(140), (11), (4), (3), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(119), (62), (54), (36), (26), (11), (4), (4), (3), (2), (2), (1)
Pubmed 12 references available
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Product details

Target Details Application Details Handling References Images
Specificity This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Purification Protein G purified
Immunogen A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].

Target Details

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Antigen
Alternative Name Nogo (RTN4 Antibody Abstract)
Background Gene Symbol: RTN4
Gene ID 57142
UniProt Q9NQC3
Pathways Neurotrophin Signaling Pathway, Regulation of Cell Size

Application Details

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Application Notes Western Blot 1:500-1:2000, Simple Western 1:1000, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:100This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27132996). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

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Product cited in:

Jin, Hu, Liu, Rana, Aguilera-Barrantes, Kong, Kumar, Wang, Gao, Wang, Duan, Shi, Song, Yang, Li, Han, Zhao, Fan, Miao: "Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel." in: Cancer letters, Vol. 419, pp. 233-244, 2019 (Sample species: Human).

Farías, Fréal, Tortosa, Stucchi, Pan, Portegies, Will, Altelaar, Hoogenraad: "Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity." in: Neuron, Vol. 102, Issue 1, pp. 184-201.e8, 2019 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Chino, Hatta, Natsume, Mizushima: "Intrinsically Disordered Protein TEX264 Mediates ER-phagy." in: Molecular cell, Vol. 74, Issue 5, pp. 909-921.e6, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Gao, Wang, Jin, Hu, Duan, Shi, Du, Song, Yang, Li, Han, Zhao, Zhang, Fan, Miao: "Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells." in: Breast cancer research : BCR, Vol. 20, Issue 1, pp. 112, 2018 Method employed by authors: Immunohistochemistry (IHC)

Kawaguchi, Tashiro, Taniguchi, Kawai, Tanaka, Okuda, Hayashi, Uchiyama: "Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1864, Issue 8, pp. 2600-2609, 2018 Method employed by authors: Western Blotting (WB), Immunohistochemistry (IHC) (Sample species: Human).

Rodríguez-Feo, Gallego-Delgado, Puerto, Wandosell, Osende: "Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells." in: Biochimica et biophysica acta, Vol. 1863, Issue 8, pp. 1985-95, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Chan, Russell, Marçal, Lee, Holden, Foster: "BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration." in: Biomacromolecules, Vol. 15, Issue 1, pp. 339-49, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Jozsef, Tashiro, Kuo, Park, Skoura, Albinsson, Rivera-Molina, Harrison, Iwakiri, Toomre, Sessa: "Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9380-95, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Teng, Rana, Afolayan, Zhao, Miao, Konduri: "Nogo-B receptor modulates angiogenesis response of pulmonary artery endothelial cells through eNOS coupling." in: American journal of respiratory cell and molecular biology, Vol. 51, Issue 2, pp. 169-77, 2014 (Sample species: Sheep (Ovine)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Wang, Zhao, North, Kong, Huang, Miao: "Expression of NgBR is highly associated with estrogen receptor alpha and survivin in breast cancer." in: PLoS ONE, Vol. 8, Issue 11, pp. e78083, 2013 (Sample species: Rabbit).

Gramolini, Kislinger, Alikhani-Koopaei, Fong, Thompson, Isserlin, Sharma, Oudit, Trivieri, Fagan, Kannan, Higgins, Huedig, Hess, Arab, Seidman, Seidman, Frey, Perry, Backx, Liu, MacLennan, Emili: "Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 519-33, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rodriguez-Feo, Hellings, Verhoeven, Moll, de Kleijn, Prendergast, Gao, van der Graaf, Tellides, Sessa, Pasterkamp: "Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 27, Issue 6, pp. 1354-60, 2007 (Sample species: Human). Further details: ELISA

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Reticulon 4 (RTN4) antibody (ABIN4340280) Immunohistochemistry-Paraffin: Nogo Antibody [ABIN43402801] - analysis of human testi...
Simple Western (SimWes) image for anti-Reticulon 4 (RTN4) antibody (ABIN4340280) Simple Western: Nogo Antibody [ABIN43402801] - Simple Western lane view shows a speci...
Western Blotting (WB) image for anti-Reticulon 4 (RTN4) antibody (ABIN4340280) Western Blot: Nogo Antibody [ABIN43402801] - analysis of Nogo A and B in A) human, B)...