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|Antigen||Reticulon 4 (RTN4) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
|12 references available|
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|Specificity||This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).|
|Purification||Protein G purified|
|Immunogen||A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].|
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|Alternative Name||Nogo (RTN4 Antibody Abstract)|
|Background||Gene Symbol: RTN4|
|Pathways||Neurotrophin Signaling Pathway, Regulation of Cell Size|
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|Application Notes||Western Blot 1:500-1:2000, Simple Western 1:1000, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:100This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27132996). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
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Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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|Product cited in:||
Jin, Hu, Liu, Rana, Aguilera-Barrantes, Kong, Kumar, Wang, Gao, Wang, Duan, Shi, Song, Yang, Li, Han, Zhao, Fan, Miao: "Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel." in: Cancer letters, Vol. 419, pp. 233-244, 2019 (Sample species: Human).
Farías, Fréal, Tortosa, Stucchi, Pan, Portegies, Will, Altelaar, Hoogenraad: "Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity." in: Neuron, Vol. 102, Issue 1, pp. 184-201.e8, 2019 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).
Chino, Hatta, Natsume, Mizushima: "Intrinsically Disordered Protein TEX264 Mediates ER-phagy." in: Molecular cell, Vol. 74, Issue 5, pp. 909-921.e6, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Gao, Wang, Jin, Hu, Duan, Shi, Du, Song, Yang, Li, Han, Zhao, Zhang, Fan, Miao: "Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells." in: Breast cancer research : BCR, Vol. 20, Issue 1, pp. 112, 2018 Method employed by authors: Immunohistochemistry (IHC)
Kawaguchi, Tashiro, Taniguchi, Kawai, Tanaka, Okuda, Hayashi, Uchiyama: "Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1864, Issue 8, pp. 2600-2609, 2018 Method employed by authors: Western Blotting (WB), Immunohistochemistry (IHC) (Sample species: Human).
Rodríguez-Feo, Gallego-Delgado, Puerto, Wandosell, Osende: "Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells." in: Biochimica et biophysica acta, Vol. 1863, Issue 8, pp. 1985-95, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Chan, Russell, Marçal, Lee, Holden, Foster: "BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration." in: Biomacromolecules, Vol. 15, Issue 1, pp. 339-49, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting
Jozsef, Tashiro, Kuo, Park, Skoura, Albinsson, Rivera-Molina, Harrison, Iwakiri, Toomre, Sessa: "Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9380-95, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Teng, Rana, Afolayan, Zhao, Miao, Konduri: "Nogo-B receptor modulates angiogenesis response of pulmonary artery endothelial cells through eNOS coupling." in: American journal of respiratory cell and molecular biology, Vol. 51, Issue 2, pp. 169-77, 2014 (Sample species: Sheep (Ovine)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)
Wang, Zhao, North, Kong, Huang, Miao: "Expression of NgBR is highly associated with estrogen receptor alpha and survivin in breast cancer." in: PLoS ONE, Vol. 8, Issue 11, pp. e78083, 2013 (Sample species: Rabbit).
Gramolini, Kislinger, Alikhani-Koopaei, Fong, Thompson, Isserlin, Sharma, Oudit, Trivieri, Fagan, Kannan, Higgins, Huedig, Hess, Arab, Seidman, Seidman, Frey, Perry, Backx, Liu, MacLennan, Emili: "Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 519-33, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting
Rodriguez-Feo, Hellings, Verhoeven, Moll, de Kleijn, Prendergast, Gao, van der Graaf, Tellides, Sessa, Pasterkamp: "Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 27, Issue 6, pp. 1354-60, 2007 (Sample species: Human). Further details: ELISA
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