anti-Human CD40 antibody for ELISA (Detection)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • dynactin 2
  • CD40 antigen
  • CD40
  • Dctn2
  • Cd40
Epitope
AA 1-193, Extracellular Domain
55
41
36
25
23
22
19
18
11
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
646
312
67
20
14
10
10
6
4
3
2
1
1
1
1
Host
Rabbit
503
213
168
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
85
50
36
24
21
17
15
14
13
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, ELISA (Detection)
580
243
205
158
112
103
103
65
47
34
19
15
13
13
9
6
6
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1997457
$ 273.90
Plus shipping costs $45.00

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 1-193, Extracellular Domain
(55), (41), (36), (25), (23), (22), (19), (18), (11), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(646), (312), (67), (20), (14), (10), (10), (6), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(503), (213), (168), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (85), (50), (36), (24), (21), (17), (15), (14), (13), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, ELISA (Detection)
(580), (243), (205), (158), (112), (103), (103), (65), (47), (34), (19), (15), (13), (13), (9), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Specificity Human CD40 / TNFRSF5 / Bp50
No cross-reactivity in ELISA with
Human CD27 / TNFRSF7
Mouse CD27 / TNFRSF7
Human CD30 / TNFRSF8
Human DR6 / TNFRSF21
Human CD137 / 4-1BB
Human TNFRSF1B / CD120b
Human HVEM / TNFRSF14
Mouse HVEM / TNFRSF14
Human RELT / TNFRSF19L
No Cross-Reactivity Human, Mouse (Murine)
Characteristics This antibody was obtained from a rabbit immunized with purified, recombinant Human CD40 extracellular domain (rh CD40, ABIN2003380 NP_001241.1, Met 1-Arg 193,.
Sterility 0.2 μm filtered
Immunogen Recombinant Human CD40 protein (ABIN2003380)
Clone 4
Isotype IgG

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
NCBI Accession NP_001241.1
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD40. The detection limit for Human CD40 is 0.0049 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months