anti-Human CD40 antibody for Intracellular Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Reactivity
Human
644
312
67
20
14
10
10
6
4
3
2
1
1
1
1
1
Host
Mouse
503
213
167
23
7
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to APC
87
85
50
35
24
21
17
15
14
13
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
580
250
213
158
109
102
99
65
49
34
18
15
13
13
9
6
6
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2658658
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2663450 ICFC PE Mouse IgG1 kappa Log in to see 5C3 5
1 ABIN2657426 FACS ICFC Alexa Fluor 488 Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2658656 FACS ICFC APC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2661710 ICFC FITC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2663451 ICFC PE Mouse IgG1 kappa Log in to see 5C3 5
1 ABIN2661711 ICFC FITC Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2658657 FACS ICFC APC Mouse IgG1 kappa Log in to see 5C3 4

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Human
(644), (312), (67), (20), (14), (10), (10), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(503), (213), (167), (23), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to APC
(87), (85), (50), (35), (24), (21), (17), (15), (14), (13), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(580), (250), (213), (158), (109), (102), (99), (65), (49), (34), (18), (15), (13), (13), (9), (6), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2658658) Images
Cross-Reactivity Rhesus Monkey
Purification The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone HB14
Isotype IgG1 kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN2658658) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN2658658) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN2658658) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD40 antibody (ABIN2658658)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Iwata, Matsushita, Horikawa, Dilillo, Yanaba, Venturi, Szabolcs, Bernstein, Magro, Williams, Hall, St Clair, Tedder: "Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells." in: Blood, Vol. 117, Issue 2, pp. 530-41, 2011

Jayakumar, Donovan, Tripathi, Ramalho-Ortigao, McDowell: "Leishmania major infection activates NF-kappaB and interferon regulatory factors 1 and 8 in human dendritic cells." in: Infection and immunity, Vol. 76, Issue 5, pp. 2138-48, 2008

Sestak, Scheiners, Wu, Hollemweguer: "Identification of anti-human CD antibodies reactive with rhesus macaque peripheral blood cells." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 21-6, 2007

Cavanagh, Boyce, Smith, Padmanabha, Filgueira, Pietschmann, Thomas: "Rheumatoid arthritis synovium contains plasmacytoid dendritic cells." in: Arthritis research & therapy, Vol. 7, Issue 2, pp. R230-40, 2005

Armengol, Juan, Lucas-Martín, Fernández-Figueras, Jaraquemada, Gallart, Pujol-Borrell: "Thyroid autoimmune disease: demonstration of thyroid antigen-specific B cells and recombination-activating gene expression in chemokine-containing active intrathyroidal germinal centers." in: The American journal of pathology, Vol. 159, Issue 3, pp. 861-73, 2001

Pound, Challa, Holder, Armitage, Dower, Fanslow, Kikutani, Paulie, Gregory, Gordon: "Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells." in: International immunology, Vol. 11, Issue 1, pp. 11-20, 1999

Foy, Aruffo, Bajorath, Buhlmann, Noelle: "Immune regulation by CD40 and its ligand GP39." in: Annual review of immunology, Vol. 14, pp. 591-617, 1996

Banchereau, Bazan, Blanchard, Brière, Galizzi, van Kooten, Liu, Rousset, Saeland: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2658658) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (APC) (ABIN2658658) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (APC)