anti-Mouse (Murine) CD40 antibody for Enzyme Immunoassay

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Epitope
Extracellular Domain
52
41
36
25
24
22
19
18
12
8
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
645
311
67
20
14
10
10
6
4
3
2
1
1
1
1
1
Host
Rat
504
212
167
23
7
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
85
50
36
24
21
17
15
14
13
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
580
250
213
158
109
101
99
65
48
34
19
15
13
13
9
6
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Pubmed
3 references available

Available images

Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (ABIN118121)
Catalog No. ABIN118121
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118122 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
1 ABIN319540 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
1 ABIN118123 EIA FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 2

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope Extracellular Domain
(52), (41), (36), (25), (24), (22), (19), (18), (12), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(645), (311), (67), (20), (14), (10), (10), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Host Rat
(504), (212), (167), (23), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (85), (50), (36), (24), (21), (17), (15), (14), (13), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(580), (250), (213), (158), (109), (101), (99), (65), (48), (34), (19), (15), (13), (13), (9), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118121) Images
Specificity Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. Clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Purification Affinity Chromatography on Protein G.
Endotoxin Level Low Endotoxin
Immunogen Extracellular domain of Mouse CD40 and the Fc portion of Human IgG1. Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Clone 01-03-23
Isotype IgG2a

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN118121) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Gene ID 21939
NCBI Accession NP_035741
UniProt P27512
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN118121) Images back to top
Application Notes Flow Cytometry: use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. ELISA. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN118121) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-CD40 antibody (ABIN118121)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." in: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, 1994

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Parry, Holman, Hasbold, Klaus: "Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes." in: European journal of immunology, Vol. 24, Issue 4, pp. 974-9, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN118121) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (Extracellular Domain) antibody (ABIN118121) Staining of mouse spleen with RAT ANTI MOUSE CD40: LOW ENDOTOXIN