anti-Mouse (Murine) CD40 antibody for ELISA

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Epitope
AA 20-193
51
37
36
24
23
22
20
18
8
7
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
629
277
75
20
15
10
9
6
4
4
3
2
2
1
1
1
1
1
Host
Rabbit
476
189
168
22
6
2
Clonality
Polyclonal
Conjugate
This CD40 antibody is un-conjugated
82
80
46
36
18
15
12
11
10
9
7
6
6
6
6
6
6
6
6
6
6
5
5
4
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
539
253
206
181
108
92
83
81
50
32
20
19
13
9
6
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN4886526
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531702 ELISA WB Rabbit IgG AA 220-269, pThr254 Log in to see Polyclonal 1
1 ABIN1533424 IHC ELISA WB Rabbit IgG AA 228-277 Log in to see Polyclonal 0
1 ABIN3183772 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN3182275 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 0
1 ABIN212959 ELISA FACS IHC IHC (fro) Rat IgG2a Extracellular Domain Log in to see 3-23 0
1 ABIN2889270 ELISA IHC WB Rabbit IgG AA 228-277 Log in to see Polyclonal 0
1 ABIN2480318 IHC (fro) FACS ELISA Rat IgG2a Extracellular Domain Log in to see 3-23 4
1 ABIN6254889 ELISA ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5960844 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5693201 ELISA WB Rabbit AA 24-193 Log in to see Polyclonal 0
1 ABIN213174 ELISA FACS IHC IHC (fro) Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN6260623 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2884429 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 0
1 ABIN600789 ELISA FACS Func IHC IHC (fro) Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN2480328 Func IHC (fro) FACS ELISA Rat IgG2a Extracellular Domain Log in to see 3-23 4
1 ABIN206090 ELISA FACS IHC IHC (fro) FITC Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN576317 ELISA FACS IHC IHC (fro) PE Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN2662168 ELISA Pacific Blue Rat IgG2a kappa Log in to see 3-23 3
1 ABIN5960845 ELISA WB Rabbit IgG pThr254 Log in to see Polyclonal 0
1 ABIN345419 ELISA FACS IHC IHC (fro) Rat IgG2a, kappa Log in to see 3-23 0

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 20-193
(51), (37), (36), (24), (23), (22), (20), (18), (8), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(629), (277), (75), (20), (15), (10), (9), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(476), (189), (168), (22), (6), (2)
Clonality
Polyclonal
Conjugate This CD40 antibody is un-conjugated
(82), (80), (46), (36), (18), (15), (12), (11), (10), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(539), (253), (206), (181), (108), (92), (83), (81), (50), (32), (20), (19), (13), (9), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB, IHC-P, ELISA in Mouse.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 5(CD40) detection. Tested with WB, IHC-P, ELISA in Mouse.
Gene Name: CD40 Molecule, TNF receptor superfamily member 5
Protein Name: Tumor necrosis factor receptor superfamily member 5
Purification Immunogen affinity purified.
Immunogen E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.
Isotype IgG
Plasmids, Primers & others

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

Synonyms: B cell associated molecule CD40 | B cell surface antigen CD40 | B-cell surface antigen CD40 | Bp50 | CD40 antigen | CD40 Molecule | CD40 protein | CD40 type II isoform | CD40L receptor | CDw40 | TNFRSF5 | TNR5 | GP39 | HIGM1 | P25942
Gene ID 21939
UniProt P27512
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 20-193) antibody (ABIN4886526) CD40/TNFRSF5 was detected in paraffin-embedded sections of mouse lymphaden tissues us...
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 20-193) antibody (ABIN4886526) Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody . Overlay hist...
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 20-193) antibody (ABIN4886526) Western blot analysis of CD40/TNFRSF5 expression in mouse spleen extract ( Lane 1). C...