anti-Mouse (Murine) CD40 antibody for Functional Studies

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Reactivity
Mouse (Murine)
686
321
74
20
15
10
9
6
4
4
3
2
2
1
1
1
1
1
Host
Rat
532
210
190
22
7
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
90
88
50
41
24
21
17
15
15
14
9
9
9
9
9
9
9
8
7
7
7
7
6
6
6
6
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
615
278
223
223
112
103
97
85
48
33
20
19
13
9
8
6
6
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1169011
$ 224.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118121 EIA Func FACS IHC (fro) Rat IgG2a Extracellular Domain Log in to see 01-03-23 3
1 ABIN370940 Func FACS IP Rat IgG2a Log in to see 1C10 6
1 ABIN370941 Func FACS IP Rat IgG2a Log in to see 1C10 6
1 ABIN1169013 Func FACS Rat IgG2a Log in to see FGK45 0
1 ABIN1169010 Func FACS Rat IgG2a Log in to see FGK45 0
1 ABIN1169012 Func FACS Rat IgG2a Log in to see FGK45 0
1 ABIN600789 ELISA FACS Func IHC IHC (fro) Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN2480328 Func IHC (fro) FACS ELISA Rat IgG2a Extracellular Domain Log in to see 3-23 4
1 ABIN2618548 Func FACS Rat IgG2a Log in to see FGK45 0
1 ABIN5690638 FACS Func Rat IgG2a Log in to see FGK45 0
1 ABIN2618547 Func FACS Rat IgG2a Log in to see FGK45 0
1 ABIN225861 Func FACS Biotin Rat IgG2a Log in to see 0
1 ABIN207919 Func FACS Rat IgG2a Log in to see 0
1 ABIN217603 ELISA FACS Func IHC IHC (fro) Rat IgG2a Extracellular Domain Log in to see 0
1 ABIN5690639 FACS Func Rat IgG2a Log in to see FGK45 0
1 ABIN5774130 Func Rat IgG2a Log in to see FGK45 0
1 ABIN2567689 Func FACS Hamster IgM kappa Log in to see HM40-3 0
1 ABIN2567688 Func FACS Hamster IgM kappa Log in to see HM40-3 0
1 ABIN2567687 Func FACS Hamster IgM kappa Log in to see HM40-3 0
1 ABIN2567686 Func FACS Hamster IgM kappa Log in to see HM40-3 0

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine)
(686), (321), (74), (20), (15), (10), (9), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rat
(532), (210), (190), (22), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(90), (88), (50), (41), (24), (21), (17), (15), (15), (14), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(615), (278), (223), (223), (112), (103), (97), (85), (48), (33), (20), (19), (13), (9), (8), (6), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN1169011) Images
Specificity Recognizes mouse CD40.
Cross-Reactivity Mouse (Murine)
Purification Purified from concentrated hybridoma tissue culture supernatant.
Purity >95 % (SDS-PAGE)
Endotoxin Level <0.01EU/μg purified protein (LAL test, Lonza).
Immunogen Recombinant mouse CD40 fusion protein.
Clone FGK45
Isotype IgG2a
Plasmids, Primers & others

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN1169011) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 belongs to the TNF-receptor superfamily and is essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of CD40-CD40L is necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.
UniProt P27512
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN1169011) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Widely used stimulatory MAb to CD40. Shown to indirectly activate natural killer (NK) cells, producing significant antitumor and antimetastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN1169011) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months

References for anti-CD40 antibody (ABIN1169011)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Greene, DeVecchio, Gould, Auletta, Heinzel: "In vivo and in vitro regulation of type I IFN synthesis by synergistic effects of CD40 and type II IFN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5995-6003, 2006

Hellings, Kasran, Bullens, Overbergh, Mathieu, Heremans, Matthys, Boon, Jorissen, Ceuppens: "IL-10- and IL-12-independent down-regulation of allergic sensitization by stimulation of CD40 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5138-44, 2006

Honda, Yanai, Negishi, Asagiri, Sato, Mizutani, Shimada, Ohba, Takaoka, Yoshida, Taniguchi: "IRF-7 is the master regulator of type-I interferon-dependent immune responses." in: Nature, Vol. 434, Issue 7034, pp. 772-7, 2005

Erlebacher, Zhang, Parlow, Glimcher: "Ovarian insufficiency and early pregnancy loss induced by activation of the innate immune system." in: The Journal of clinical investigation, Vol. 114, Issue 1, pp. 39-48, 2004

McGregor, Schoenberger, Green: "CD154 is a negative regulator of autoaggressive CD8+ T cells in type 1 diabetes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9345-50, 2004

Turner, Rakhmilevich, Burdelya, Neal, Imboden, Sondel, Yu: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, 2001

Mauri, Mars, Londei: "Therapeutic activity of agonistic monoclonal antibodies against CD40 in a chronic autoimmune inflammatory process." in: Nature medicine, Vol. 6, Issue 6, pp. 673-9, 2000

Rolink, Andersson, Melchers: "Characterization of immature B cells by a novel monoclonal antibody, by turnover and by mitogen reactivity." in: European journal of immunology, Vol. 28, Issue 11, pp. 3738-48, 1998