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|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This EGFR antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling Images
|Specificity||Recognizes human Epidermal Growth Factor Receptor.|
|Purification||Protein G purified|
Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.
Type of Immunogen: Recombinant protein
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling Images back to top
|Alternative Name||EGFR (EGFR Antibody Abstract)|
Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
|Research Area||Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Approved: ELISA, ICC, IF, IHC-P (5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
|Buffer||0.15 M PBS, pH 7.4. No preservatives added.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top