anti-Human EGFR antibody for Immunofluorescence

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
N-Term
151
128
67
63
58
56
56
54
53
52
48
48
43
41
40
36
33
31
30
29
27
25
25
25
22
19
18
18
18
18
16
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
8
7
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2428
851
684
29
24
18
17
15
15
8
3
2
2
1
1
1
1
Host
Rabbit
1467
859
79
44
9
5
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
104
82
73
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1484
685
629
540
516
272
263
225
125
90
65
56
43
24
19
14
12
11
11
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2854848
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.712676 ABIN2801210 IC IF IP IHC WB Rabbit C-Term, pTyr1016 Log in to see Polyclonal 0
11.712676 ABIN2704752 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
11.712676 ABIN2706078 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.712676 ABIN2706075 IC IF IHC WB Rabbit pSer693 Log in to see Polyclonal 0
11.712676 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
11.712676 ABIN1995321 IHC (fro) IF IHC (p) ELISA Rabbit IgG AA 1-645 Log in to see 043 0
11.712676 ABIN1532331 IF IHC ELISA WB Rabbit IgG AA 986-1035 Log in to see Polyclonal 0
11.712676 ABIN1532195 IF IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 0
11.712676 ABIN1531312 IF IHC ELISA WB Rabbit IgG AA 986-1035, pTyr1016 Log in to see Polyclonal 0
11.712676 ABIN1531177 IF ELISA WB Rabbit IgG AA 651-700, pThr678 Log in to see Polyclonal 0
11.712676 ABIN1531178 IF IHC ELISA WB Rabbit IgG AA 661-710, pThr693 Log in to see Polyclonal 0
11.712676 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
11.712676 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
11.712676 ABIN1995313 FACS IF IF/ICC Mouse IgG1 AA 1-645 Log in to see 08 0
11.712676 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
11.712676 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450 0
11.712676 ABIN5668056 FACS IF IHC in vivo Mouse IgG1, kappa Extracellular Domain Log in to see 7A7 0
11.712676 ABIN2852742 IC IF IP WB Mouse Log in to see 0
11.712676 ABIN2852743 IC IF IHC WB Mouse Log in to see 0
11.712676 ABIN2957555 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope N-Term
(151), (128), (67), (63), (58), (56), (56), (54), (53), (52), (48), (48), (43), (41), (40), (36), (33), (31), (30), (29), (27), (25), (25), (25), (22), (19), (18), (18), (18), (18), (16), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2428), (851), (684), (29), (24), (18), (17), (15), (15), (8), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1467), (859), (79), (44), (9), (5), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(104), (82), (73), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1484), (685), (629), (540), (516), (272), (263), (225), (125), (90), (65), (56), (43), (24), (19), (14), (12), (11), (11), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Pig (Porcine)
Cross-Reactivity (Details) Mouse (94 %), Rat (100 %), Pig (100 %)
Characteristics Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian))
EGFR antibody [N1], N-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EGFR. The exact sequence is proprietary.
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Isoform 2: Secreted
Molecular Weight 134 kDa65
Gene ID 1956
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: A431 , HCT-116

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (N-Term) antibody (ABIN2854848) WB Image EGFR antibody detects EGFR protein by western blot analysis. Whole cell extr...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor Receptor (EGFR) (N-Term) antibody (ABIN2854848) IHC-P Image Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using ...
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (N-Term) antibody (ABIN2854848) WB Image EGFR antibody detects EGFR protein by western blot analysis. Whole cell extr...
Immunofluorescence (IF) image for anti-Epidermal Growth Factor Receptor (EGFR) (N-Term) antibody (ABIN2854848) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EGFR, ...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor Receptor (EGFR) (N-Term) antibody (ABIN2854848) IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, us...