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|Antigen||Growth Hormone 1 (GH1) Antibodies|
|Reactivity||Cat (Feline), Dog (Canine), Human, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This Growth Hormone 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Growth Hormone 1 AntibodyTarget Details Growth Hormone 1 Application Details Handling Images
|Specificity||This antibody recognizes human Growth Hormone (GH), which acts to control body growth and metabolic activity. In Western blotting a band of approximately 20 kDa is seen using lysates of human pituitary gland.|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Growth hormone from ovine pituitary.|
Target Details Growth Hormone 1Product Details anti-Growth Hormone 1 Antibody Application Details Handling Images back to top
|Alternative Name||Somatotropin / Growth Hormone / GH1 (GH1 Antibody Abstract)|
|Background||Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.Synonyms: Growth hormone 1, HGH, Pituitary growth hormone|
|Pathways||NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process|
Application DetailsProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Handling Images back to top
Western blot: 1/50-1/500. Immunofluorescence: 1/10-1/100. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500-1/4000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Images back to top
|Buffer||Phosphate buffered saline containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Storage in frost-free freezers is not recommended.|