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|Antigen||Interleukin 1 alpha (IL1A) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This IL1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-IL1A AntibodyTarget Details IL1A Application Details Handling Images
|Specificity||This antibody reacts with Rat Interleukin 1 alpha|
|Immunogen||Recombinant rat IL-1 alpha|
Target Details IL1AProduct Details anti-IL1A Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-1 alpha / IL-1A (IL1A Antibody Abstract)|
|Background||Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1|
|Pathways||NF-kappaB Signaling, Autophagy|
Application DetailsProduct Details anti-IL1A Antibody Target Details IL1A Handling Images back to top
Immunohistochemistry: we recommend use of 1 μg per ml as a starting dilution. Paraffin sections may require pretreatment like Proteinase K or Trypsin predigestion toimprove staining. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1alpha (50 pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required. ELISA: To detect rIL-1 alpha by direct ELISA (using 100 ml/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant rIL-1 alpha. Western blot: To detect rIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rIL-1 alpha is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1A Antibody Target Details IL1A Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|