We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Interleukin 1, beta (IL1B) Antibodies|
|Conjugate||This IL1B antibody is un-conjugated Alternatives|
Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Neutralization (Neut)
|Supplier||Log in to see|
Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling Images
|Purification||Protein A or G purified|
|Immunogen||The immunogen for this antibody was IL1b.|
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling Images back to top
|Alternative Name||IL-1 beta/il-1F2 (IL1B Antibody Abstract)|
|Background||Gene Symbol: IL1B|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
|Application Notes||Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Blocking/Neutralizing 50-200 Molar excessThe CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1beta (IL-1beta) in combination with the biotin CRM57 antibody for detection and recombinant human IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1beta bioactivity. The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 μg/million cells/100 μL staining volume. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 μL per million cells 100 μL blood.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Aqueous, may contain carrier protein/stabilizer.
Buffer contains: 0.09 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 4°C. Do not freeze.|