anti-Human IL1B antibody for Blocking Peptide

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
Reactivity
Human
541
196
138
39
31
21
20
15
14
13
12
9
8
4
4
2
2
2
1
1
Host
Mouse
438
360
17
14
10
7
6
2
2
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
103
46
33
24
19
15
13
12
10
10
8
8
8
8
8
8
8
4
4
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Neutralization (Neut)
566
400
216
145
112
73
65
59
44
42
34
32
23
16
14
5
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4324368
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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(541), (196), (138), (39), (31), (21), (20), (15), (14), (13), (12), (9), (8), (4), (4), (2), (2), (2), (1), (1)
Host Mouse
(438), (360), (17), (14), (10), (7), (6), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(103), (46), (33), (24), (19), (15), (13), (12), (10), (10), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Neutralization (Neut)
(566), (400), (216), (145), (112), (73), (65), (59), (44), (42), (34), (32), (23), (16), (14), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Purification Protein A or G purified
Immunogen The immunogen for this antibody was IL1b.
Clone CRM56
Isotype IgG1
Plasmids, Primers & others

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Gene Symbol: IL1B
Gene ID 3553
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Blocking/Neutralizing 50-200 Molar excessThe CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1beta (IL-1beta) in combination with the biotin CRM57 antibody for detection and recombinant human IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1beta bioactivity. The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 μg/million cells/100 μL staining volume. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 μL per million cells 100 μL blood.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous, may contain carrier protein/stabilizer.
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.