We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Protein Kinase A, alpha (PRKACA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PRKACA antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-PRKACA AntibodyTarget Details PRKACA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PKAalpha/beta cat protein.(region surrounding Lys17)|
|Purification||Affinity Chromatography using epitope-specific immunogen.|
|Plasmids, Primers & others|
Target Details PRKACAProduct Details anti-PRKACA Antibody Application Details Handling Images back to top
|Alternative Name||PRKACA (PRKACA Antibody Abstract)|
|Background||The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93 % amino acid sequence similarity), whereas Cgamma displays 83 % and 79 % similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha|
|Molecular Weight||approx. 38 kDa|
|Pathways||NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism|
Application DetailsProduct Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top