anti-Cow (Bovine) TGFA antibody for Flow Cytometry

Recommended TGFA Antibody (supplied by: Log in to see )

Antigen
Transforming Growth Factor, alpha (TGFA) Antibodies
  • TFGA
  • LOC100009482
  • TGF alpha
  • TGFA
  • wa-1
  • wa1
  • RATTGFAA
  • TGFAA
  • NV15606
  • si:ch211-10e8.7
  • transforming growth factor alpha
  • transforming growth factor alpha L homeolog
  • transforming growth factor-alpha
  • transforming growth factor, alpha
  • TGFA
  • TGF-A
  • Tgfa
  • tgfa.L
  • LOC100121300
  • LOC100009150
  • tgfa
Epitope
C-Term
177
92
16
8
7
5
5
3
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
469
192
188
118
102
55
9
3
3
2
1
Host
Mouse
321
159
12
4
2
Clonality
Monoclonal
Conjugate
This TGFA antibody is un-conjugated
31
18
15
14
10
8
8
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
299
271
217
170
159
150
99
96
45
21
17
13
13
5
4
4
2
2
1
1
Supplier
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Catalog No. ABIN3026161
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6171396 FACS IF IHC (f) CF®594 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171376 FACS IF IHC (f) CF®640R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171382 FACS IF IHC (f) CF®555 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171372 FACS IF IHC (f) CF®405S Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171386 FACS IF IHC (f) CF®568 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171392 FACS IF IHC (f) CF®680R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171374 FACS IF IHC (f) CF405M Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171378 FACS IF IHC (f) CF®543 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171384 FACS IF IHC (f) CF®660R Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171390 FACS IF IHC (f) CF®680 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171394 FACS IF IHC (f) CF®488A Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171388 FACS IF IHC (f) CF®770 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171380 FACS IF IHC (f) CF®647 Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171407 FACS IF IHC (f) Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171401 FACS IF IHC (f) Biotin Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171399 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171405 FACS IF IHC (f) PerCP Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171406 FACS IF IHC (f) PE Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171398 FACS IF IHC (f) APC Mouse IgG1, kappa C-Term Log in to see MF9 0
1 ABIN6171409 FACS IF IHC (f) Mouse IgG1, kappa C-Term Log in to see MF9 0

General

Antigen Transforming Growth Factor, alpha (TGFA) Antibodies
Epitope C-Term
(177), (92), (16), (8), (7), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(469), (192), (188), (118), (102), (55), (9), (3), (3), (2), (1)
Host Mouse
(321), (159), (12), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TGFA antibody is un-conjugated
(31), (18), (15), (14), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(299), (271), (217), (170), (159), (150), (99), (96), (45), (21), (17), (13), (13), (5), (4), (4), (2), (2), (1), (1)
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Product Details anti-TGFA Antibody

Target Details TGFA Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clone MF9
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details TGFA

Product Details anti-TGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name TGF alpha (TGFA Antibody Abstract)
Background TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Gene ID 7039
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-TGFA Antibody Target Details TGFA Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-TGFA Antibody Target Details TGFA Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-TGFA Antibody Target Details TGFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibody (ABIN3026161) IHC testing of FFPE human testicular carcinoma with TGF alpha antibody