anti-Mouse (Murine) FOXL2 antibody for Immunohistochemistry

Recommended FOXL2 Antibody (supplied by: Log in to see )

Antigen
Forkhead Box L2 (FOXL2) Antibodies
  • BPES
  • BPES1
  • PFRK
  • PINTO
  • POF3
  • AU045128
  • Pfrk
  • FOXL2
  • zgc:136549
  • foxl2
  • FoxL2
  • foxL2
  • forkhead box L2
  • forkhead box L2a
  • forkhead box L2 S homeolog
  • forkhead transcription factor L2
  • foxl2 protein
  • fork-head box L2 transcription factor
  • FOXL2
  • Foxl2
  • foxl2a
  • foxl2
  • foxl2.S
  • LOC751846
  • foxL2
Epitope
C-Term
39
22
21
20
10
8
4
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
123
48
35
10
8
7
5
5
3
2
2
1
Host
Goat
104
17
4
Clonality
Polyclonal
Conjugate
This FOXL2 antibody is un-conjugated
6
6
6
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
95
47
39
23
22
13
11
8
4
3
3
2
2
1
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Catalog No. ABIN185040
$ 429.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN2562656 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN2740959 IF IHC IHC (p) WB Rabbit IgG AA 1-14 Log in to see Polyclonal 0
1 ABIN4951078 ELISA IHC WB Goat Ig Fraction Log in to see Polyclonal 0

General

Antigen Forkhead Box L2 (FOXL2) Antibodies
Epitope C-Term
(39), (22), (21), (20), (10), (8), (4), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(123), (48), (35), (10), (8), (7), (5), (5), (3), (2), (2), (1)
Host Goat
(104), (17), (4)
Clonality
Polyclonal
Conjugate This FOXL2 antibody is un-conjugated
(6), (6), (6), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(95), (47), (39), (23), (22), (13), (11), (8), (4), (3), (3), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-FOXL2 Antibody

Target Details FOXL2 Application Details Handling References for anti-FOXL2 antibody (ABIN185040) Images
Purpose FOXL2 / BPES
Blocking Peptide Blocking peptide for this product available: ABIN368927
Sequence DSKTGALHSR LDL
Predicted Reactivity Human, Mouse, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.
Plasmids, Primers & others

Target Details FOXL2

Product Details anti-FOXL2 Antibody Application Details Handling References for anti-FOXL2 antibody (ABIN185040) Images back to top
Antigen
Alternative Name FOXL2 (FOXL2 Antibody Abstract)
Background FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3
Gene ID 668
NCBI Accession NP_075555
Pathways Nuclear Hormone Receptor Binding, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FOXL2 Antibody Target Details FOXL2 Handling References for anti-FOXL2 antibody (ABIN185040) Images back to top
Application Notes

DS WB Results: Approx 50 kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8 kDa according to Human NP_075555.1 and 38.9 kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.

DS IHC Results: In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only

Handling

Product Details anti-FOXL2 Antibody Target Details FOXL2 Application Details References for anti-FOXL2 antibody (ABIN185040) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-FOXL2 antibody (ABIN185040)

Product Details anti-FOXL2 Antibody Target Details FOXL2 Application Details Handling Images back to top
Product cited in:

Padua, Fox, Jiang, Morse, Tevosian: "Simultaneous gene deletion of gata4 and gata6 leads to early disruption of follicular development and germ cell loss in the murine ovary." in: Biology of reproduction, Vol. 91, Issue 1, pp. 24, 2014

Blount, Schmidt, Justice, Vale, Fischer, Bilezikjian: "FoxL2 and Smad3 coordinately regulate follistatin gene transcription." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7631-45, 2009

Background publications

Crisponi, Deiana, Loi, Chiappe, Uda, Amati, Bisceglia, Zelante, Nagaraja, Porcu, Ristaldi, Marzella, Rocchi, Nicolino, Lienhardt-Roussie, Nivelon, Verloes, Schlessinger, Gasparini, Bonneau, Cao, Pilia: "The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome." in: Nature genetics, Vol. 27, Issue 2, pp. 159-66, 2001

Images

Product Details anti-FOXL2 Antibody Target Details FOXL2 Application Details Handling References for anti-FOXL2 antibody (ABIN185040) back to top
Supplier Images
Western Blotting (WB) image for anti-Forkhead Box L2 (FOXL2) (C-Term) antibody (ABIN185040) ABIN185040 staining (1.5µg/ml) of Human Ovary extracts (RIPA buffer, 30µg total prote...