anti-Hamster Nuclear Receptor Subfamily 1, Group H, Member 3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Nuclear Receptor Subfamily 1, Group H, Member 3 Antibody

Antigen
Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) Antibodies
  • NR1H3
  • LXR-a
  • LXRA
  • RLD-1
  • AU018371
  • LXR
  • RLD1
  • Unr1
  • LXRalpha
  • LXR-alpha
  • im:7141239
  • wu:fd59b01
  • zgc:113339
  • zgc:158542
  • nuclear receptor subfamily 1 group H member 3
  • nuclear receptor subfamily 1, group H, member 3
  • NR1H3
  • nr1h3
  • Nr1h3
Epitope
C-Term
14
13
10
9
9
8
8
7
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
145
82
77
10
8
8
6
5
5
4
3
3
2
2
2
2
1
1
1
Host
Rabbit
134
15
6
Clonality
Polyclonal
Conjugate
Un-conjugated
10
8
5
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
131
58
25
18
10
9
8
6
4
3
3
3
2
2
1
1
Options

Available images

Catalog No. ABIN6741573
$ 727.83
Plus shipping costs $45.00

General

Antigen Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) Antibodies
Epitope C-Term
(14), (13), (10), (9), (9), (8), (8), (7), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(145), (82), (77), (10), (8), (8), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(134), (15), (6)
Clonality
Polyclonal
Conjugate Un-conjugated
(10), (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(131), (58), (25), (18), (10), (9), (8), (6), (4), (3), (3), (3), (2), (2), (1), (1)
Supplier

Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human NR1H3 / LXR Alpha
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Guinea pig, Chicken, Zebrafish (100%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details

Product details Application Details Handling Images back to top
Antigen
Alternative Name NR1H3 / LXR Alpha (NR1H3 Antibody Abstract)
Background Name/Gene ID: NR1H3
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1
Gene ID 10062
NCBI Accession NP_005684
UniProt Q13133
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C

Application Details

Product details Target Details Handling Images back to top
Application Notes Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product details Target Details Application Details Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Images

Product details Target Details Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (C-Term) antibody (ABIN6741573) Human Colon (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody ABIN46221...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (C-Term) antibody (ABIN6741573) Human Small Intestine (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (C-Term) antibody (ABIN6741573) Human Liver (formalin-fixed, paraffin-embedded) stained with NR1H3 antibody ABIN46221...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (C-Term) antibody (ABIN6741573) Anti-NR1H3 / LXR Alpha antibody IHC of human colon. Immunohistochemistry of formalin-...