anti-Mouse (Murine) Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) antibody for Immunohistochemistry

Recommended Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) Antibody

Antigen
Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
  • GR
  • gcr
  • grl
  • xGR
  • gccr
  • gr
  • zgc:113038
  • NR3C1
  • GCL
  • LOC100302444
  • Gcr
  • Grl
  • Grl-1
  • Grl1
  • GRL
  • GCCR
  • GCR
  • NO
  • GR-A
  • nr3c1
  • nuclear receptor subfamily 3 group C member 1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1
  • glucocorticoid receptor
  • NR3C1
  • nr3c1
  • LOC100302444
  • Nr3c1
Reactivity
Mouse (Murine), Rat (Rattus), Human
290
150
134
8
7
6
5
5
4
4
3
1
1
1
Host
Rabbit
249
57
5
Clonality
Polyclonal
Conjugate
Un-conjugated
23
17
10
7
6
6
6
6
6
6
6
6
3
3
3
3
3
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
225
119
105
63
53
39
35
29
27
17
4
3
2
1
1
Options

Available images

Catalog No. ABIN3023091
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
14.587412 ABIN6673506 IHC WB Rabbit IgG Polyclonal 3
13.926594 ABIN268501 BP ChIP FACS GS ICC IF IHC IHC (p) IP Neut WB Mouse IgG2 BuGR2 8
13.087412 ABIN2782262 IHC WB Rabbit N-Term Polyclonal 1
13.087412 ABIN6263722 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
13.087412 ABIN2957584 IC IF IP IHC WB Rabbit Polyclonal 0
13.087412 ABIN2430185 IHC ELISA WB Rabbit IgG Polyclonal 0
10.087412 ABIN2706239 IHC WB Rabbit pSer211 Polyclonal 0
10.087412 ABIN2777208 IHC WB Rabbit N-Term Polyclonal 0
10.087412 ABIN1873953 IF IHC WB Rabbit IgG Polyclonal 0
10.087412 ABIN6569150 IHC WB Rabbit IgG Polyclonal 0
10.087412 ABIN2971701 IHC WB Rabbit Center, pSer211 Polyclonal 0
10.087412 ABIN2431461 IHC ELISA Rabbit IgG Polyclonal 0
10.087412 ABIN2426013 IHC ELISA Rabbit IgG Polyclonal 0
10.087412 ABIN2423539 IHC ELISA Rabbit IgG Polyclonal 0
10 ABIN2888599 ELISA IF IHC IHC (p) WB Rabbit IgG AA 181-230 Polyclonal 0
9.426594 ABIN152786 ChIP ICC IF IHC (p) IP IHC WB Rabbit IgG Polyclonal 1
9.426594 ABIN152763 ChIP ICC IF IHC (p) IHC WB Rabbit IgG Polyclonal 1
7 ABIN2888632 ELISA IHC IHC (p) WB Rabbit IgG AA 201-250 Polyclonal 0
7 ABIN1173541 ICC IHC WB Rabbit IgG AA 622-755 Polyclonal 0
7 ABIN2973608 IHC WB Rabbit IgG Polyclonal 0

General

Antigen Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(290), (150), (134), (8), (7), (6), (5), (5), (4), (4), (3), (1), (1), (1)
Host Rabbit
(249), (57), (5)
Clonality
Polyclonal
Conjugate Un-conjugated
(23), (17), (10), (7), (6), (6), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(225), (119), (105), (63), (53), (39), (35), (29), (27), (17), (4), (3), (2), (1), (1)
Pubmed 3 references available
Supplier

Product details

Target Details Application Details Handling References Images
Purification Affinity purification
Immunogen Recombinant protein of human NR3C1
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name NR3C1 (NR3C1 Antibody Abstract)
Background Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors. GR is composed of several conserved structural elements, including a carboxy-terminal ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization signals, a central zinc-finger-containing DNA-binding domain, and an amino-terminal variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an inactive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific DNA sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes. It was demonstrated that GR-mediated transcriptional activation is modulated by phosphorylation. Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, receptor stability, and receptor subcellular localization. Indeed Ser211 of human GR is phosphorylated to a greater extent in the presence of hormone, and biochemical fractionation studies following hormone treatment indicate that Ser211-phosphorylated GR is found in the nucleus. Thus, Ser211 phosphorylation is a biomarker for activated GR in vivo. An added layer of complexity to GR signaling lies in the ability of multiple isoforms to be generated through both alternative splicing and the use of alternative translation intiation start sites, thus increasing the repertoire of functional signaling homo- and heterodimers.
Synonyms: GCCR, GCR, GR, GRL
Gene ID 2908
UniProt P04150
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process

Application Details

Product details Target Details Handling References Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

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Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

References

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Product cited in:

Liu, Chen, Pei, Zhang, Zou, Xiao, Zhou, Chen, Wang: "Decreased H3K9ac level of StAR mediated testicular dysplasia induced by prenatal dexamethasone exposure in male offspring rats." in: Toxicology, Vol. 408, pp. 1-10, 2018 Method employed by authors: Western Blotting (WB)

Zhang, Yao, Xiao, Lan, Yu, Zhang, Jiang, Yang, Pei, Li, Rong, Hu, Li, Xu: "Administration of dexamethasone protects mice against ischemia/reperfusion induced renal injury by suppressing PI3K/AKT signaling." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2366-75, 2014 Method employed by authors: Western Blotting (WB)

Zhang, Li, Wang, Han, Xiao, Lan, Li, Xu, Yao: "Glucocorticoid receptor agonist dexamethasone attenuates renal ischemia/reperfusion injury by up-regulating eNOS/iNOS." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 516-520, 2014 Method employed by authors: Western Blotting (WB)

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN3023091) anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody
Western Blotting (WB) image for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN3023091) Western blot analysis of extracts of various cell lines, using NR3C1 antibody.