anti-Human Splicing Factor 1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Splicing Factor 1 Antibody (supplied by: Log in to see )

Antigen
Splicing Factor 1 (SF1) Antibodies
  • BBP
  • D11S636
  • MBBP
  • ZCCHC25
  • ZFM1
  • ZNF162
  • BB094781
  • CW17R
  • MZFM
  • WBP4
  • Zfp162
  • CG5836
  • Dmel\\CG5836
  • cg5836
  • dSF1
  • p70
  • SF1
  • sf1
  • wu:fc09f06
  • znf162
  • MWD22.25
  • MWD22_25
  • splicing factor 1
  • Splicing factor 1
  • splicing factor-like protein
  • splicing factor 1 S homeolog
  • SF1
  • Sf1
  • sf1
  • AT5G51300
  • sf1.S
Epitope
AA 11-30, N-Term
17
12
10
10
7
7
7
6
5
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
106
57
29
19
17
16
14
12
11
10
8
7
3
2
1
1
Host
Rabbit
93
13
Clonality
Polyclonal
Conjugate
This Splicing Factor 1 antibody is un-conjugated
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
100
61
51
31
26
6
6
5
2
2
1
Options
Supplier
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Catalog No. ABIN3044091
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2628220 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 11-30 Log in to see Polyclonal 0
1 ABIN3032719 ICC IHC (fro) IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Splicing Factor 1 (SF1) Antibodies
Epitope AA 11-30, N-Term
(17), (12), (10), (10), (7), (7), (7), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(106), (57), (29), (19), (17), (16), (14), (12), (11), (10), (8), (7), (3), (2), (1), (1)
Host Rabbit
(93), (13)
Clonality
Polyclonal
Conjugate This Splicing Factor 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(100), (61), (51), (31), (26), (6), (6), (5), (2), (2), (1)
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Product Details anti-Splicing Factor 1 Antibody

Target Details Splicing Factor 1 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence DFPSKKRKRS RWNQDTMEQK
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: splicing factor 1
Protein Name: Splicing factor 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1(11-30aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences.
Isotype IgG
Plasmids, Primers & others

Target Details Splicing Factor 1

Product Details anti-Splicing Factor 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SF1 (SF1 Antibody Abstract)
Background SF1(splicing factor 1), also known as zinc finger protein 162(ZFM162), ZNF162, Transcription factor ZFM1, ZFM1, D11S636, Mammalian branch point-binding protein(BBP, mBBP), Zinc finger gene in MEN1 locus, is a protein that in humans is encoded by the SF1 gene. It is predicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.

Synonyms: BBP antibody|D11S636 antibody|Mammalian branch point-binding protein mBBP antibody|OTTHUMP00000069749 antibody|OTTHUMP00000069750 antibody|OTTHUMP00000069752 antibody|splicing factor 1 antibody|Transcription factor ZFM1 antibody|ZFM1 antibody|Zinc finger gene in MEN1 locus antibody|Zinc finger protein 162 antibody|ZNF162 antibody
UniProt Q15637
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location

Application Details

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibody (ABIN3044091) Anti-splicing factor 1 antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibody (ABIN3044091) Anti-splicing factor 1 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibody (ABIN3044091) anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibody (ABIN3044091) Anti-splicing factor 1 antibody, IHC(F) IHC(F): Rat Spleen Tissue