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|Antigen||Splicing Factor 1 (SF1) Antibodies|
|Epitope||AA 11-30, N-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Splicing Factor 1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Splicing Factor 1 AntibodyTarget Details Splicing Factor 1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: splicing factor 1
Protein Name: Splicing factor 1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1(11-30aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences.|
|Plasmids, Primers & others|
Target Details Splicing Factor 1Product Details anti-Splicing Factor 1 Antibody Application Details Handling Images back to top
|Alternative Name||SF1 (SF1 Antibody Abstract)|
SF1(splicing factor 1), also known as zinc finger protein 162(ZFM162), ZNF162, Transcription factor ZFM1, ZFM1, D11S636, Mammalian branch point-binding protein(BBP, mBBP), Zinc finger gene in MEN1 locus, is a protein that in humans is encoded by the SF1 gene. It is predicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
Synonyms: BBP antibody|D11S636 antibody|Mammalian branch point-binding protein mBBP antibody|OTTHUMP00000069749 antibody|OTTHUMP00000069750 antibody|OTTHUMP00000069752 antibody|splicing factor 1 antibody|Transcription factor ZFM1 antibody|ZFM1 antibody|Zinc finger gene in MEN1 locus antibody|Zinc finger protein 162 antibody|ZNF162 antibody
|Pathways||Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location|
Application DetailsProduct Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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