anti-Human Splicing Factor 1 antibody for Immunoprecipitation

Recommended Splicing Factor 1 Antibody (supplied by: Log in to see )

Antigen
Splicing Factor 1 (SF1) Antibodies
  • BBP
  • D11S636
  • MBBP
  • ZCCHC25
  • ZFM1
  • ZNF162
  • BB094781
  • CW17R
  • MZFM
  • WBP4
  • Zfp162
  • CG5836
  • Dmel\\CG5836
  • cg5836
  • dSF1
  • p70
  • SF1
  • sf1
  • wu:fc09f06
  • znf162
  • MWD22.25
  • MWD22_25
  • splicing factor 1
  • Splicing factor 1
  • splicing factor-like protein
  • splicing factor 1 S homeolog
  • SF1
  • Sf1
  • sf1
  • AT5G51300
  • sf1.S
Epitope
AA 1-111
17
13
10
10
7
7
6
6
5
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
106
56
30
19
17
16
14
12
11
10
8
7
3
2
1
1
Host
Mouse
94
12
Clonality
Monoclonal
Conjugate
This Splicing Factor 1 antibody is un-conjugated
2
2
2
2
2
2
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
100
60
50
30
27
7
6
4
3
2
1
Options
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Catalog No. ABIN394721
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.259117 ABIN563411 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 1-110, partial Log in to see 2E12 0
1 ABIN442548 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN1934094 IP Rabbit IgG AA 225-275 Log in to see Polyclonal 0
1 ABIN1934095 IP WB Rabbit IgG AA 25-75 Log in to see Polyclonal 0

General

Antigen Splicing Factor 1 (SF1) Antibodies
Epitope AA 1-111
(17), (13), (10), (10), (7), (7), (6), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(106), (56), (30), (19), (17), (16), (14), (12), (11), (10), (8), (7), (3), (2), (1), (1)
Host Mouse
(94), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Splicing Factor 1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(100), (60), (50), (30), (27), (7), (6), (4), (3), (2), (1)
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Product Details anti-Splicing Factor 1 Antibody

Target Details Splicing Factor 1 Application Details Handling Images
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 2E12
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details Splicing Factor 1

Product Details anti-Splicing Factor 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SF1 (SF1 Antibody Abstract)
Background

Other Names: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162

Gene Description: splicing factor 1

Molecular Weight 68330 DA
Gene ID 7536
NCBI Accession NP_004621, NP_001171501, NP_001171502, NP_973724, NP_973726, NP_973727
UniProt Q15637
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location

Application Details

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Handling Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Restrictions For Research Use only

Handling

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

Product Details anti-Splicing Factor 1 Antibody Target Details Splicing Factor 1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Immunoperoxidase of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded hu...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (Image 2)
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) SF1 monoclonal antibody (M01), clone 2E12. Western Blot analysis of SF1 expression in...
Western Blotting (WB) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Western Blot analysis of SF1 expression in transfected 293T cell line by SF1 monoclon...
Immunohistochemistry (IHC) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Immunoperoxidase of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded hu...
Immunofluorescence (IF) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Immunofluorescence of monoclonal antibody to SF1 on HeLa cell . [antibody concentrati...
Immunoprecipitation (IP) image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody and ...
ELISA image for anti-Splicing Factor 1 (SF1) (AA 1-111) antibody (ABIN394721) Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a captur...