We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Checkpoint Kinase 1 (CHEK1) Antibodies|
|Epitope||AA 192-241 Alternatives|
|Reactivity||Bat, Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This CHEK1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CHEK1 AntibodyTarget Details CHEK1 Application Details Handling Images
|Specificity||Human CHEK1 / CHK1|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Dog, Horse (100%) Bovine, Pig, Guinea pig, Chicken (92%).|
Synthetic peptide located between aa192-241 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Zebra finch (100%), Hamster, Bovine, Pig, Opossum, Guinea pig, Chicken, Platypus (92%), Stickleback (85%).
Type of Immunogen: Synthetic peptide
Target Details CHEK1Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
|Alternative Name||CHEK1 / CHK1 (CHEK1 Antibody Abstract)|
Name/Gene ID: CHEK1
Family: Protein Kinase
Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
|Reconstitution||After adding water, will consist of PBS buffer with 2 % sucrose|
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top