anti-Human CHEK2 antibody for ELISA

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • cds1
  • chek2
  • chk2
  • hucds1
  • lfs2
  • pp1425
  • rad53
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • checkpoint kinase 2 L homeolog
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • chek2.L
Epitope
AA 1-544
55
53
38
38
19
19
19
17
15
15
10
10
8
7
7
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
526
238
216
28
25
16
13
8
5
4
2
1
1
1
1
1
1
Host
Mouse
396
131
8
1
1
Clonality
Monoclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
10
9
7
7
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
443
164
153
114
106
66
57
47
39
10
6
4
2
1
1
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Catalog No. ABIN393759
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.942492 ABIN564867 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-543, full length Log in to see 4B7 0
1 ABIN2869319 ELISA ICC IHC WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 0
1 ABIN1531263 IHC ELISA WB Rabbit IgG AA 361-410, pThr387 Log in to see Polyclonal 0
1 ABIN1531498 IF ELISA WB Rabbit IgG AA 356-405, pThr383 Log in to see Polyclonal 0
1 ABIN1532281 IHC ELISA WB Rabbit IgG AA 361-410 Log in to see Polyclonal 0
1 ABIN1531802 ELISA WB Rabbit IgG AA 486-535, pSer516 Log in to see Polyclonal 0
1 ABIN1532512 ELISA WB Rabbit IgG AA 356-405 Log in to see Polyclonal 0
1 ABIN1531803 IHC ELISA WB Rabbit IgG AA 35-84, pThr68 Log in to see Polyclonal 0
1 ABIN1532833 IHC ELISA WB Rabbit IgG AA 35-84 Log in to see Polyclonal 0
1 ABIN129576 ELISA WB Rabbit IgG AA 64-73, pThr68 Log in to see Polyclonal 1
1 ABIN2878986 ELISA IHC IHC (p) WB Rabbit IgG AA 35-84 Log in to see Polyclonal 0
1 ABIN1843154 ELISA IF/ICC IHC WB Mouse IgG2b AA 481-531 Log in to see 0
1 ABIN466738 IF IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 0
1 ABIN6280579 ELISA IHC (p) WB Rabbit IgG AA 10-90, pThr68 Log in to see Polyclonal 0
1 ABIN2888620 ELISA IHC WB Rabbit IgG AA 361-410 Log in to see Polyclonal 0
1 ABIN2888765 ELISA WB Rabbit IgG AA 356-405 Log in to see Polyclonal 0
1 ABIN2888383 ELISA WB Rabbit IgG pSer516 Log in to see Polyclonal 0
1 ABIN1532832 ELISA WB Rabbit IgG AA 486-535 Log in to see Polyclonal 0
1 ABIN965887 IF IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 4
1 ABIN1724714 ICC IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 3

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 1-544
(55), (53), (38), (38), (19), (19), (19), (17), (15), (15), (10), (10), (8), (7), (7), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(526), (238), (216), (28), (25), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(396), (131), (8), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHEK2 antibody is un-conjugated
(10), (10), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(443), (164), (153), (114), (106), (66), (57), (47), (39), (10), (6), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN393759) Images
Specificity CHEK2 (AAH04207, 1 a.a. ~ 544 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: CHEK2 (AAH04207.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 4B7
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling References for anti-CHEK2 antibody (ABIN393759) Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background

Other Names: Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2, CHEK2, CDS1, CHK2, RAD53

Gene Description: CHK2 checkpoint homolog (S. pombe)

Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Molecular Weight 60915 DA
Gene ID 11200
NCBI Accession NP_001005735, NP_001244316, NP_009125, NP_665861
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling References for anti-CHEK2 antibody (ABIN393759) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details References for anti-CHEK2 antibody (ABIN393759) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-CHEK2 antibody (ABIN393759)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Background publications

Ho-Pun-Cheung, Assenat, Bascoul-Mollevi, Bibeau, Boissière-Michot, Thezenas, Cellier, Azria, Rouanet, Senesse, Ychou, Lopez-Crapez: "A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 437-43, 2011

Guénard, Pedneault, Ouellette, Labrie, Simard, , Durocher: "Evaluation of the contribution of the three breast cancer susceptibility genes CHEK2, STK11, and PALB2 in non-BRCA1/2 French Canadian families with high risk of breast cancer." in: Genetic testing and molecular biomarkers, Vol. 14, Issue 4, pp. 515-26, 2010

Konstantinova, Kadiyska, Sokolova, Kaneva, Mirchev, Savov, Aleksandrova, Nedin, Kostadinov, Damyanov, Kremensky, Mitev: "CHEK2 I157T and colorectal cancer in Bulgaria." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 2, pp. 314-7, 2010

Monsees, Kraft, Chanock, Hunter, Han: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010

Evans, Ahmed, Bayliss, Howard, Lalloo, Wallace: "BRCA1, BRCA2 and CHEK2 c.1100 delC mutations in patients with double primaries of the breasts and/or ovaries." in: Journal of medical genetics, Vol. 47, Issue 8, pp. 561-6, 2010

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN393759) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (Image 2)
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) CHEK2 monoclonal antibody (M01), clone 4B7. Western Blot analysis of CHEK2 expression...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) CHEK2 monoclonal antibody (M01), clone 4B7 Western Blot analysis of CHEK2 expression ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded ...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentrat...
ELISA image for anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (ABIN393759) Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture...