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|Antigen||Cyclin G1 (CCNG1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cyclin G1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cyclin G1 AntibodyTarget Details Cyclin G1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Cyclin G protein. (region surrounding Ile194)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details Cyclin G1Product Details anti-Cyclin G1 Antibody Application Details Handling Images back to top
|Alternative Name||Cyclin G1 (CCNG1 Antibody Abstract)|
|Background||Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. The G1 to S transition, however, appears to be controlled by the G1 cyclins. Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. Cyclin G2 shares 53 % amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.Synonyms: CCNG, CCNG1, CYCG1, Cyclin-G, Cyclin-G1|
|Molecular Weight||approx. 29 kDa|
|Research Area||Cell Cycle|
Application DetailsProduct Details anti-Cyclin G1 Antibody Target Details Cyclin G1 Handling Images back to top
ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin G1 Antibody Target Details Cyclin G1 Application Details Images back to top
|Buffer||PBS, pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Cyclin G1 Antibody Target Details Cyclin G1 Application Details Handling back to top