Human CXCL7 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all CXCL7 (PPBP) products
- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
- Binding Specificity
- AA 59-128
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL7 in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: E.coli
Immunogen sequence: A59-D128 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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PPBP (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
- Alternative Name
- PPBP (PPBP Products)
- Synonyms
- 2400003M24Rik Matched Antibody Pair, AI854500 Matched Antibody Pair, CTAP3 Matched Antibody Pair, CTAPIII Matched Antibody Pair, Cxcl7 Matched Antibody Pair, LA-PF4 Matched Antibody Pair, LDGF Matched Antibody Pair, MDGF Matched Antibody Pair, NAP-2 Matched Antibody Pair, NAP-2-L1 Matched Antibody Pair, Scyb7 Matched Antibody Pair, TGB Matched Antibody Pair, TGB1 Matched Antibody Pair, THBGB1 Matched Antibody Pair, b-TG1 Matched Antibody Pair, beta-TG Matched Antibody Pair, B-TG1 Matched Antibody Pair, Beta-TG Matched Antibody Pair, CTAP-III Matched Antibody Pair, CXCL7 Matched Antibody Pair, PBP Matched Antibody Pair, SCYB7 Matched Antibody Pair, TC1 Matched Antibody Pair, TC2 Matched Antibody Pair, THBGB Matched Antibody Pair, Nap-2 Matched Antibody Pair, CTAPIII/NAP-2 Matched Antibody Pair, Platelet basic protein Matched Antibody Pair, pro-platelet basic protein Matched Antibody Pair, scyb7 Matched Antibody Pair, Ppbp Matched Antibody Pair, PPBP Matched Antibody Pair
- Background
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Protein Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .
Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Synonyms: Platelet basic protein,PBP,C-X-C motif chemokine 7,Leukocyte-derived growth factor,LDGF,Macrophage-derived growth factor,MDGF,Small-inducible cytokine B7,Connective tissue-activating peptide III,CTAP-III,LA-PF4,Low-affinity platelet factor IV,TC-2,Connective tissue-activating peptide III(1-81),CTAP-III(1-81),Beta-thromboglobulin,Beta-TG,Neutrophil-activating peptide 2(74),NAP-2(74),Neutrophil-activating peptide 2(73),NAP-2(73),Neutrophil-activating peptide 2,NAP-2,TC-1,Neutrophil-activating peptide 2(1-66),NAP-2(1-66),Neutrophil-activating peptide 2(1-63),NAP-2(1-63),PPBP,CTAP3, CXCL7, SCYB7, TGB1, THBGB1,
Full Gene Name: Platelet basic protein
Cellular Localisation: Secreted. - UniProt
- P02775
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