Human TNFRSF14/HVEM EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all HVEM (TNFRSF14) products
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Binding Specificity
- AA 39-202
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects TNFRSF14/HVEM in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: NSO
Immunogen sequence: L39-V202 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Sequence similarities: Contains 3 TNFR-Cys repeats.
Tissue Specificity: Widely expressed, with the highest expression in lung, spleen and thymus.
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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TNFRSF14 (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
TNFRSF14 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
TNFRSF14 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
TNFRSF14 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
TNFRSF14 (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Rabbit, Mouse
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Alternative Name
- TNFRSF14 (TNFRSF14 Products)
- Synonyms
- ATAR Matched Antibody Pair, CD270 Matched Antibody Pair, HVEA Matched Antibody Pair, HVEM Matched Antibody Pair, LIGHTR Matched Antibody Pair, TR2 Matched Antibody Pair, Atar Matched Antibody Pair, HveA Matched Antibody Pair, Hvem Matched Antibody Pair, Tnfrs14 Matched Antibody Pair, TNF receptor superfamily member 14 Matched Antibody Pair, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Matched Antibody Pair, Tnfrsf14 Matched Antibody Pair, TNFRSF14 Matched Antibody Pair
- Target Type
- Viral Protein
- Background
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Protein Function: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .
Background: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.
Synonyms: Tumor necrosis factor receptor superfamily member 14,Herpes virus entry mediator A,Herpesvirus entry mediator A,HveA,Tumor necrosis factor receptor-like 2,TR2,CD270,TNFRSF14,HVEA, HVEM,UNQ329/PRO509,
Full Gene Name: Tumor necrosis factor receptor superfamily member 14
Cellular Localisation: Membrane, Single-pass type I membrane protein. - UniProt
- Q92956
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