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PERK Antibodies

(Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

PERK antibodies (also known as EIF2AK3 antibodies) are valuable tools in scientific research for exploring the aspects of PERK's role in cellular biology and its implications for various diseases. These antibodies are used in a variety of experimental techniques to detect, quantify, and manipulate PERK protein levels and activity. These are the most common research applications of PERK antibodies:

Western Blotting (WB): Scientists use PERK antibodies in Western blotting experiments to assess the expression and activation status of PERK in different cellular conditions. PERK antibodies can detect changes in PERK protein levels, as well as the phosphorylation status of PERK and its downstream target, eIF2α, which provides insights into the cellular response to ER stress.
Immunoprecipitation (IP): PERK antibodies are employed for immunoprecipitation assays to isolate and purify PERK protein complexes from cell lysates. This technique helps researchers study protein-protein interactions involving PERK and identify its binding partners, shedding light on the molecular mechanisms of PERK function.
Immunofluorescence (IF) and Immunohistochemistry (IHC):Immunofluorescence and immunohistochemistry techniques utilize PERK antibodies to visualize the subcellular localization of PERK within cells or tissues.These methods are crucial for understanding where PERK is active and how it is distributed within specific cell types or tissues under different conditions.

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101 results

PERK antibody (AA 973-1114)

EIF2AK3 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Detection of Recombinant EIF2aK3, Mouse using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Detection of EIF2aK3 in Mouse Testis Tissue using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Detection of EIF2aK3 in Human Hela cell using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

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Detection of EIF2aK3 in Mouse Stomach Tissue using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Detection of EIF2aK3 in Mouse Kidney Tissue using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Detection of EIF2aK3 in Mouse Pancreas Tissue using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

Detection of EIF2aK3 in Mouse Cerebrum Tissue using Polyclonal Antibody to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3)

8 images

Catalog No. ABIN7441241

Datasheet as PDF

PERK antibody (pThr982)

EIF2AK3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24 h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12.

ABIN6277783 at 1/100 staining human liver carcinoma tissue sections by IHC-P.

ABIN6277783 staining Hela by IF/ICC.

ABIN6277783 staining A549 cells by ICC/IF.

Western blot analysis of p-PERK (Thr 982) using 293 whole cell lysates

5 images

3 references

Catalog No. ABIN6255756

Datasheet as PDF

PERK antibody (pThr980)

EIF2AK3 Reactivity: Human, Mouse, Rat, Dog, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (ABIN744713) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (ABIN744713) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat pancreas tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PERK (Thr980) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

3 images

14 references

Catalog No. ABIN744713

Datasheet as PDF

PERK antibody (C-Term)

EIF2AK3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Western blot analysis of PERK expression in Hela cells.

ABIN6268851 staining lovo cells by ICC/IF.

Western blot analysis of PERK expression in mouse brain

ABIN6268851 at 1/100 staining Human breast cancer tissue by IHC-P.

4 images

3 references

Catalog No. ABIN6264143

Datasheet as PDF

PERK antibody (AA 1001-1116)

EIF2AK3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-PERK Polyclonal Antibody, Unconjugated (ABIN740715) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

U-87MG cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature.

3 images

2 references

Catalog No. ABIN740715

Datasheet as PDF

PERK antibody (AA 115-330)

EIF2AK3 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

IHC image of ABIN7152141 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system.

IHC image of ABIN7152141 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system.

Immunofluorescence staining of Hela cells with ABIN7152141 at 1:100, counter-stained with DAPI.

3 images

Catalog No. ABIN7152141

Datasheet as PDF

PERK antibody (N-Term)

EIF2AK3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB15305 unconjugated

Western blot analysis of PERK Antibody (N-term ) (ABIN392617 and ABIN2842134) in 293 cell line lysates (35 μg/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term ) (ABIN392617 and ABIN2842134) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

2 images

1 reference

Catalog No. ABIN392617

Datasheet as PDF

PERK antibody (AA 665-764)

EIF2AK3 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

Western Blot detection against Immunogen (37 KDa) .

EIF2AK3 polyclonal antibody (A01), Lot # 060525JCS1 Western Blot analysis of EIF2AK3 expression in PC-12

EIF2AK3 polyclonal antibody (A01).

3 images

Catalog No. ABIN564080

Datasheet as PDF

PERK antibody (N-Term)

EIF2AK3 Reactivity: Human WB, IHC, IF, IP, IC Host: Rabbit Polyclonal unconjugated

Western blot analysis of PERK expression in HeLa (A) whole cell lysates.

Immunofluorescent analysis of PERK staining in HeLa cells.

Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section.

Immunoprecipitation of PERK from 0.

4 images

Catalog No. ABIN7297604

Datasheet as PDF

PERK antibody (pThr981)

EIF2AK3 Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western Blotting (WB) image for anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr981) antibody (ABIN3182929)

Western Blot (WB) analysis of specific cells using antibody diluted at 1:1000.

2 images

Catalog No. ABIN3182929

Datasheet as PDF

PERK antibody

EIF2AK3 Reactivity: Human ELISA Host: Mouse Monoclonal 5G5 unconjugated

Western blot analysis using EIF2AK3 mAb against HEK293 (1) and EIF2AK3(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate.

1 image

2 references

Catalog No. ABIN969093

Datasheet as PDF

PERK antibody (AA 665-764)

EIF2AK3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 7B10 unconjugated

Western Blot detection against Immunogen (36.

Detection limit for recombinant GST tagged EIF2AK3 is 0.

2 images

Catalog No. ABIN1774836

Datasheet as PDF

PERK antibody (AA 148-175)

EIF2AK3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western blot analysis of PERK antibody and 293 lysate

IHC analysis of FFPE human hepatocarcinoma tissue stained with PERK antibody

Western blot analysis of PERK antibody and 293 lysate

3 images

Catalog No. ABIN3030887

Datasheet as PDF

PERK antibody (pThr981)

EIF2AK3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western Blot analysis of 453 AD293 cells using Phospho-PERK (T981) Polyclonal Antibody

1 image

Catalog No. ABIN7182401

Datasheet as PDF

PERK antibody (N-Term)

EIF2AK3 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Western Blot analysis of Hela cells using PERK Polyclonal Antibody

1 image

Catalog No. ABIN7188942

Datasheet as PDF

PERK antibody (pThr981)

EIF2AK3 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr982) antibody (left)or the same antibody preincubated with blocking peptide (right).

1 image

Catalog No. ABIN7138887

Datasheet as PDF

PERK antibody (AA 530-850)

EIF2AK3 Reactivity: Human, Mouse, Rat WB Host: Mouse Monoclonal 1385CT582-4-39 unconjugated

All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: PC-12 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse liver lysate Lysates/proteins at 20 μg per lane.

1 image

Catalog No. ABIN6243642

Datasheet as PDF

PERK antibody

EIF2AK3 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Mouse Monoclonal 3C3 unconjugated

Lane 1: A549, Lane 2: PC-12, Lane 3: mouse brain, Lane 4: mouse liver cell lysate at 20 µg per lane, probed with bsm-51385M EIF2AK3 (1385CT582.

1 image

Catalog No. ABIN4909079

Datasheet as PDF

PERK antibody (pThr980) (AbBy Fluor® 647)

EIF2AK3 Reactivity: Human, Mouse, Rat, Dog, Pig WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal AbBy Fluor® 647

1 reference

Catalog No. ABIN878623

Datasheet as PDF

PERK antibody (AA 73-102)

EIF2AK3 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

Anti-EIF2AK3 / PERK antibody IHC staining of human kidney.

1 image

Catalog No. ABIN1978701

Datasheet as PDF

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