anti-Cow (Bovine) Proprotein Convertase Subtilisin/kexin Type 2 antibody for Western Blotting

Recommended Proprotein Convertase Subtilisin/kexin Type 2 Antibody (supplied by: Log in to see )

Antigen
Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
  • NEC 2
  • NEC-2
  • NEC2
  • PC2
  • SPC2
  • PCSK2
  • 6330411F23Rik
  • AI839700
  • Nec-2
  • Nec2
  • Phpp-2
  • pc2
  • nec2
  • spc2
  • pcsk2
  • pcsk2-A
  • proprotein convertase subtilisin/kexin type 2
  • proprotein convertase subtilisin/kexin type 2 L homeolog
  • PCSK2
  • pcsk2
  • Pcsk2
  • pcsk2.L
Epitope
AA 351-400
15
9
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
66
33
27
5
2
2
2
1
1
1
1
1
Host
Rabbit
60
6
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
49
14
14
13
13
12
7
6
3
3
1
1
Supplier
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Catalog No. ABIN611197
$ 551.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4.8023243 ABIN2788115 WB Rabbit Middle Region Log in to see Polyclonal 0

General

Antigen Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
Epitope AA 351-400
(15), (9), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(66), (33), (27), (5), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(60), (6), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(49), (14), (14), (13), (13), (12), (7), (6), (3), (3), (1), (1)
Pubmed 3 references available
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Product details

Target Details Application Details Handling References Images
Specificity Human PCSK2
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Pig (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa351-400 of human PCSK2 (Q5REC2, NP_002585). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Frog, Salmon, Stickleback, Medaka, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Target Details

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Antigen
Alternative Name PCSK2 (PCSK2 Antibody Abstract)
Background Name/Gene ID: PCSK2
Subfamily: Serine S8
Family: Protease

Synonyms: PCSK2, Proprotein convertase 2, SPC2, NEC 2, NEC-2, Neuroendocrine convertase 2, PC2, KEX2-like endoprotease 2, NEC2, Prohormone convertase 2
Gene ID 5126
NCBI Accession NP_002585
UniProt P16519
Pathways Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity

Application Details

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Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

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Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References

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Product cited in:

Discher, Bishopric, Wu, Peterson, Webster: "Hypoxia regulates beta-enolase and pyruvate kinase-M promoters by modulating Sp1/Sp3 binding to a conserved GC element." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 26087-93, 1998

Feo, Antona, Barbieri, Passantino, Calì, Giallongo: "Transcription of the human beta enolase gene (ENO-3) is regulated by an intronic muscle-specific enhancer that binds myocyte-specific enhancer factor 2 proteins and ubiquitous G-rich-box binding factors." in: Molecular and cellular biology, Vol. 15, Issue 11, pp. 5991-6002, 1995

Giallongo, Venturella, Oliva, Barbieri, Rubino, Feo: "Structural features of the human gene for muscle-specific enolase. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA." in: European journal of biochemistry / FEBS, Vol. 214, Issue 2, pp. 367-74, 1993

Images

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Supplier Images
 image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 351-400) antibody (ABIN611197) anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 351-400) antibody