anti-Rat (Rattus) Proprotein Convertase Subtilisin/kexin Type 2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Proprotein Convertase Subtilisin/kexin Type 2 Antibody (supplied by: Log in to see )

Antigen
Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
  • NEC 2
  • NEC-2
  • NEC2
  • PC2
  • SPC2
  • PCSK2
  • 6330411F23Rik
  • AI839700
  • Nec-2
  • Nec2
  • Phpp-2
  • pc2
  • nec2
  • spc2
  • pcsk2
  • pcsk2-A
  • proprotein convertase subtilisin/kexin type 2
  • proprotein convertase subtilisin/kexin type 2 L homeolog
  • PCSK2
  • pcsk2
  • Pcsk2
  • pcsk2.L
Epitope
AA 110-125, N-Term
15
8
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
66
34
27
6
3
3
3
2
2
2
2
2
1
1
Host
Rabbit
60
6
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
14
14
13
12
12
7
6
3
3
1
1
Options
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Catalog No. ABIN3044078
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1409726 IHC (p) WB HRP Rabbit IgG AA 255-290 Log in to see Polyclonal 0
1 ABIN1385997 IF (p) IHC (p) WB Rabbit IgG AA 255-290 Log in to see Polyclonal 1
1 ABIN1391833 IHC (p) WB Biotin Rabbit IgG AA 255-290 Log in to see Polyclonal 0
1 ABIN2626611 IHC IHC (p) WB Rabbit IgG AA 110-125 Log in to see Polyclonal 0
1 ABIN3032331 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) Antibodies
Epitope AA 110-125, N-Term
(15), (8), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(66), (34), (27), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(60), (6), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(49), (14), (14), (13), (12), (12), (7), (6), (3), (3), (1), (1)
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Product details

Target Details Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2(PCSK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Sequence GYRDINEIDI NMNDPL
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2(PCSK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: proprotein convertase subtilisin/kexin type 2
Protein Name: Neuroendocrine convertase 2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Proprotein Convertase 2(110-125aa GYRDINEIDINMNDPL), identical to the related mouse and rat sequences.
Isotype IgG

Target Details

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Antigen
Alternative Name PCSK2 (PCSK2 Antibody Abstract)
Background PCSK2(Proprotein Convertase, Subtilisin/Kexin-Type, 2), also known as PC2 or NEC2, is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. Seidah et al.(1991) mapped the NEC2 gene to human chromosome 20p11.2-p11.1. Ohagi et al.(1992) observed expression of the chloramphenicol acetyltransferase reporter fusions containing the putative promoter region in insulinoma cells but not in hepatoma cells, consistent with the known tissue-specific pattern of expression of the PC2 gene. Analysis of the level of CAT activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for PC2 promoter activity.

Synonyms: KEX2 like endoprotease 2 antibody|KEX2-like endoprotease 2 antibody|NEC 2 antibody|NEC2 antibody|NEC2_HUMAN antibody|Neuroendocrine convertase 2 antibody|PC2 antibody|PCSK2 antibody|Prohormone convertase 2 antibody|Proprotein convertase 2 antibody|Proprotein convertase subtilisin/kexin type 2 antibody|SPC2 antibody
UniProt P16519
Pathways Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity

Application Details

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Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for PCSK2 is approximately 10 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

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Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 110-125), (N-Term) antibody (ABIN3044078) anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 110-125), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 110-125), (N-Term) antibody (ABIN3044078) Anti-Proprotein Convertase 2 antibody, IHC(P) IHC(P): Rat Pancreas Tissue
Western Blotting (WB) image for anti-Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2) (AA 110-125), (N-Term) antibody (ABIN3044078) Anti-Proprotein Convertase 2 antibody, Western blottingAll lanes: Anti Proprotein Con...