NEDD8 Activating Enzyme E1 Subunit 1 (NAE1) (C-Term) Peptide
NAE1
Reactivity: Mouse, Human
Host: Synthetic
BP, WB
Catalog No. ABIN1541277
$160.46
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100 μg
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Quick Overview for NEDD8 Activating Enzyme E1 Subunit 1 (NAE1) (C-Term) Peptide (ABIN1541277)
Target
NAE1
(NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))
Origin
Mouse, Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- C-Term
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Sequence
- ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Nae1 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))
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Background
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Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Alias Symbols: 59 kDa, Appbp1
Protein Size: 534 -
Molecular Weight
- 60 kDa
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Gene ID
- 234664
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NCBI Accession
- NM_144931, NP_659180
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UniProt
- Q8VBW6
Target
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