NEDD8 Activating Enzyme E1 Subunit 1 (NAE1) (C-Term) Peptide
Quick Overview for NEDD8 Activating Enzyme E1 Subunit 1 (NAE1) (C-Term) Peptide (ABIN8106257)
Target
Origin
Source
Application
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Protein Region
- C-Term
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Purpose
- Nae1 Peptide - C-terminal region
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Sequence
- ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This is a synthetic peptide designed for use in combination with anti-Nae1 Antibody (ARP59379_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications. Please inquire for more details.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 µL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Buffer
- Lyophilized powder
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20 °C. Avoid repeat freeze-thaw cycles.
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- NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))
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Background
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Background Information: Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Alternative Symbols: 59 kDa, Appbp1
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Molecular Weight
- 60kDa
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Gene ID
- 234664
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NCBI Accession
- NP_659180
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UniProt
- Q8VBW6
Target
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