anti-Human RFWD2 antibody for Immunocytochemistry

Recommended RFWD2 Antibody (supplied by: Log in to see )

Antigen
Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
  • COP1
  • RNF200
  • AI316802
  • C80879
  • Cop1
  • RGD1304773
  • RFWD2
  • cop1
  • rnf200
  • zgc:163067
  • ring finger and WD repeat domain 2
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
  • ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog
  • RFWD2
  • Rfwd2
  • rfwd2
  • rfwd2.L
Reactivity
Human, Mouse (Murine)
80
36
7
4
4
3
3
3
2
2
1
1
1
1
Host
Rabbit
76
4
2
Clonality
Polyclonal
Conjugate
This RFWD2 antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
80
52
8
5
4
3
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Available images

Catalog No. ABIN6259497
$ 390.77
Plus shipping costs $45.00

General

Antigen Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) Antibodies
Reactivity Human, Mouse (Murine)
(80), (36), (7), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(76), (4), (2)
Clonality
Polyclonal
Conjugate This RFWD2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(80), (52), (8), (5), (4), (3)
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Product Details anti-RFWD2 Antibody

Target Details RFWD2 Application Details Handling Images
Specificity RFWD2 Antibody detects endogenous levels of total RFWD2
Cross-Reactivity Human, Mouse (Murine)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide
Isotype IgG
Plasmids, Primers & others

Target Details RFWD2

Product Details anti-RFWD2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RFWD2 (RFWD2 Antibody Abstract)
Background

Description: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

Gene: RFWD2

Molecular Weight 80 kDa
Gene ID 64326
UniProt Q8NHY2
Pathways Photoperiodism

Application Details

Product Details anti-RFWD2 Antibody Target Details RFWD2 Handling Images back to top
Application Notes WB 1:500~1:1000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-RFWD2 Antibody Target Details RFWD2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) antibody (ABIN6259497) Western blot analysis of extracts from LOVO cells, using RFWD2 antibody.
Western Blotting (WB) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) antibody (ABIN6259497) Western blot analysis of extracts from Rat brain lysate, using RFWD2 antibody.
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) antibody (ABIN6259497) ABIN6275092 staining Hela cells by IF/ICC. The sample were fixed with PFA and permea...