anti-Human Cyclin A antibody for Enzyme Immunoassay

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • ccn1
  • ccna
  • ccna1
  • CCN1
  • CCNA
  • AA408589
  • Ccn-1
  • Ccn1
  • Ccna
  • CycA2
  • Cyca
  • cb671
  • chunp6924
  • wu:fi36f03
  • CYCA
  • Cyclin-A2
  • ccna2
  • Cyclin-A
  • cyclinA
  • cyclin A2
  • cyclin-A2 pseudogene
  • cyclin A2 L homeolog
  • cyclin A2 S homeolog
  • ccna2
  • CCNA2
  • LOC100352785
  • Ccna2
  • ccna2.L
  • ccna2.S
Epitope
N-Term
21
18
6
5
5
4
3
3
3
2
1
1
1
1
1
Reactivity
Human
194
101
68
33
21
21
19
19
5
4
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
162
67
1
Clonality
Polyclonal
Conjugate
This Cyclin A antibody is un-conjugated
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
189
80
63
54
50
42
36
30
28
5
4
4
2
1
1
1
Supplier
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Catalog No. ABIN359544
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359545 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 2
1 ABIN265377 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN5556726 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN2718920 EIA WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Cyclin A2 (CCNA2) Antibodies
Epitope N-Term
(21), (18), (6), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Human
(194), (101), (68), (33), (21), (21), (19), (19), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(162), (67), (1)
Clonality
Polyclonal
Conjugate This Cyclin A antibody is un-conjugated
(8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(189), (80), (63), (54), (50), (42), (36), (30), (28), (5), (4), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling References for anti-Cyclin A antibody (ABIN359544) Images
Specificity This antibody reacts to Cyclin A (CCNA2).
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human CCNA2.
Isotype Ig Fraction

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling References for anti-Cyclin A antibody (ABIN359544) Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Gene ID 890, 9606
UniProt P20248
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling References for anti-Cyclin A antibody (ABIN359544) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details References for anti-Cyclin A antibody (ABIN359544) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Cyclin A antibody (ABIN359544)

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling Images back to top
Background publications

Tsang, Wang, Chen, Sánchez, Dynlacht: "SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression." in: The Journal of cell biology, Vol. 178, Issue 4, pp. 621-33, 2007

Henglein, Chenivesse, Wang, Eick, Bréchot: "Structure and cell cycle-regulated transcription of the human cyclin A gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5490-4, 1994

Wang, Chenivesse, Henglein, Bréchot: "Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma." in: Nature, Vol. 343, Issue 6258, pp. 555-7, 1990

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling References for anti-Cyclin A antibody (ABIN359544) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin A2 (CCNA2) (N-Term) antibody (ABIN359544) Western blot analysis of anti-CCNA2(N-term) Pab in HL60 cell line lysates (35ug/lane)...