anti-Rat (Rattus) MTOR antibody for Immunocytochemistry

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Reactivity
Fish, Human, Mouse (Murine), Rat (Rattus)
407
221
211
16
8
6
6
6
6
5
4
3
1
1
1
1
Host
Rabbit
355
49
3
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
10
10
9
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
294
155
127
69
58
52
33
18
16
8
8
7
3
3
2
2
2
1
1
Supplier
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Catalog No. ABIN6263424
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
1 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN6256211 ELISA ICC IF IHC WB Rabbit IgG pSer2481 Log in to see Polyclonal 1
1 ABIN2625789 ICC IF WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1915272 ICC IF IP PLA Rabbit IgG AA 200-250 Log in to see Polyclonal 0
1 ABIN2858081 ICC IF IHC WB Rabbit pSer2481 Log in to see Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Reactivity Fish, Human, Mouse (Murine), Rat (Rattus)
(407), (221), (211), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1), (1)
Host Rabbit
(355), (49), (3)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(10), (10), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(294), (155), (127), (69), (58), (52), (33), (18), (16), (8), (8), (7), (3), (3), (2), (2), (2), (1), (1)
Pubmed 7 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN6263424) Images
Specificity MTOR Antibody detects endogenous levels of total mTOR
Cross-Reactivity Fish, Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human mTOR
Isotype IgG
Plasmids, Primers & others

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN6263424) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background

Description: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).

Gene: MTOR

Molecular Weight 289kDa
Gene ID 2475
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN6263424) Images back to top
Application Notes WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN6263424) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-MTOR antibody (ABIN6263424)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Song, Zhang, Sun, Li, Chen, Xiao, Xing: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, 2018

Huang, Qi, He, Xu: "Omeprazole promotes carcinogenesis of fore-stomach in mice with co-stimulation of nitrosamine." in: Oncotarget, Vol. 8, Issue 41, pp. 70332-70344, 2018

Li, Li, Zeng, Yang, Liu, Zhang, Song, Yao, Ma, Li, Wang, Wei: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, 2018

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, 2018

Zheng, Feng, Jiang, Wu, Tang, Kuang, Zeng, Zhou, Liu: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, 2018

Sun, Wang, Song: "Long non‑coding RNA SENCR alleviates the inhibitory effects of rapamycin on human umbilical vein endothelial cells." in: Molecular medicine reports, Vol. 18, Issue 2, pp. 1405-1414, 2018

He, Cao, Guo, Li, Shang, Liu, Xie, Xu, Liu: "Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia." in: Free radical biology & medicine, Vol. 103, pp. 165-176, 2017

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN6263424) back to top
Supplier Images
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody (ABIN6263424) Western blot analysis of mTOR expression in HeLa whole cell lysates,The lane on the l...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody (ABIN6263424) ABIN6269252 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody (ABIN6263424) mTOR Antibody for IHC in human prostate tissue
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody (ABIN6263424) Western blot analysis of extracts from Mouse spleen, using mTOR Antibody. Lane 1 was ...