anti-Rat (Rattus) MTOR antibody for Immunocytochemistry

Description: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).

Gene: MTOR

Song, Zhang, Sun, Li, Chen, Xiao, Xing: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, 2018

Huang, Qi, He, Xu: "Omeprazole promotes carcinogenesis of fore-stomach in mice with co-stimulation of nitrosamine." in: Oncotarget, Vol. 8, Issue 41, pp. 70332-70344, 2018

Li, Li, Zeng, Yang, Liu, Zhang, Song, Yao, Ma, Li, Wang, Wei: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, 2018

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, 2018

Zheng, Feng, Jiang, Wu, Tang, Kuang, Zeng, Zhou, Liu: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, 2018

Sun, Wang, Song: "Long non‑coding RNA SENCR alleviates the inhibitory effects of rapamycin on human umbilical vein endothelial cells." in: Molecular medicine reports, Vol. 18, Issue 2, pp. 1405-1414, 2018

He, Cao, Guo, Li, Shang, Liu, Xie, Xu, Liu: "Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia." in: Free radical biology & medicine, Vol. 103, pp. 165-176, 2017