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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN151707 | ICC IF IP IHC PLA WB | Rabbit | AA 200-250 | Log in to see | Polyclonal | 4 | |||
1 | ABIN447030 | ICC IF IHC (p) IHC WB | Rabbit | Log in to see | Polyclonal | 1 | ||||
1 | ABIN2625789 | ICC IF WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
1 | ABIN1915272 | ICC IF IP PLA | Rabbit | IgG | AA 200-250 | Log in to see | Polyclonal | 0 | ||
1 | ABIN328657 | ICC IP WB | Rabbit | AA 200-250 | Log in to see | Polyclonal | 0 | |||
1 | ABIN2858081 | ICC IF IHC WB | Rabbit | pSer2481 | Log in to see | Polyclonal | 0 |
General |
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Antigen | Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies |
Epitope | C-Term Alternatives |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This MTOR antibody is un-conjugated Alternatives |
Application |
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-MTOR AntibodyTarget Details MTOR Application Details Handling Images |
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Characteristics |
Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase)) mTOR antibody [C3], C-term |
Purification | Purified by antigen-affinity chromatography. |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary. |
Isotype | IgG |
Target Details MTORProduct Details anti-MTOR Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | mTOR (FRAP1 Antibody Abstract) |
Background |
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side |
Molecular Weight | 289 kDa |
Gene ID | 2475 |
Pathways | PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events |
Application DetailsProduct Details anti-MTOR Antibody Target Details MTOR Handling Images back to top |
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Application Notes | Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000* |
Comment |
Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T |
Restrictions | For Research Use only |
HandlingProduct Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer | 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative. |
Preservative | ProClin |
Precaution of Use | This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | -20 °C |
Storage Comment | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
ImagesProduct Details anti-MTOR Antibody Target Details MTOR Application Details Handling back to top |
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Supplier Images |
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