anti-Rat (Rattus) MTOR antibody for Immunohistochemistry

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pSer2448
65
30
28
23
21
19
18
15
15
12
8
7
7
6
6
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
409
214
206
16
8
6
6
6
6
5
4
3
1
1
1
Host
Rabbit
356
49
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
10
10
9
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
290
155
123
75
58
52
39
18
16
8
7
7
3
3
2
2
2
1
Supplier
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Catalog No. ABIN153493
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.312915 ABIN1531910 IHC ELISA WB Rabbit IgG AA 2415-2464, pSer2448 Log in to see Polyclonal 1
9.312915 ABIN1532464 IHC ELISA WB Rabbit IgG AA 2447-2496 Log in to see Polyclonal 0
9.312915 ABIN1531447 IF IHC ELISA Rabbit IgG AA 2447-2496, pSer2481 Log in to see Polyclonal 0
9.312915 ABIN1532942 IHC ELISA WB Rabbit IgG AA 2415-2464 Log in to see Polyclonal 0
9.312915 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
9.312915 ABIN2879019 ELISA IHC IHC (p) WB Rabbit IgG AA 2447-2496 Log in to see Polyclonal 0
9.312915 ABIN2878963 ELISA IHC IHC (p) WB Rabbit IgG AA 2415-2464 Log in to see Polyclonal 0
9.312915 ABIN2879300 IF IHC IHC (p) WB Rabbit Ser2442 Log in to see Polyclonal 0
9.312915 ABIN2470319 IF IHC ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal 0
9.312915 ABIN2431685 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
9.312915 ABIN2426243 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
1 ABIN3198213 IHC WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Log in to see Polyclonal 0
1 ABIN2888475 ELISA IHC IHC (p) WB Rabbit IgG pSer2448 Log in to see Polyclonal 0
1 ABIN214561 ELISA IF IHC IHC (p) WB Rabbit AA 2440-2457 Log in to see Polyclonal 0
1 ABIN1842801 IHC WB Rabbit pSer2448 Log in to see Polyclonal 0
1 ABIN1842805 IF/ICC IHC WB Rabbit pSer2481 Log in to see Polyclonal 0
1 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal 0
1 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pSer2448
(65), (30), (28), (23), (21), (19), (18), (15), (15), (12), (8), (7), (7), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(409), (214), (206), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1)
Host Rabbit
(356), (49), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(10), (10), (9), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Electron Microscopy (EM), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(290), (155), (123), (75), (58), (52), (39), (18), (16), (8), (7), (7), (3), (3), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN153493) Images
Specificity Reactivity occurs with phosphorylated mTOR from human derived tissues and cells.
Purification Immunogen affinity purified
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2440-2457 of human mTOR.
Isotype IgG
Plasmids, Primers & others

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN153493) Images back to top
Antigen
Alternative Name MTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Gene Symbol: MTOR
Gene ID 2475
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN153493) Images back to top
Application Notes Western Blot 1:500-1:2000, ELISA 1:100 - 1:2000, Immunohistochemistry 5 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Electron Microscopy 1:10000 - 1:100000Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN153493) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-MTOR antibody (ABIN153493)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Zhou, Yue, Chan, Tsui, Fung, Sun, Pu, Lau: "Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer." in: Biochemical pharmacology, Vol. 142, pp. 58-70, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Lisi, Stigliano, Lauriola, Navarra, Dello Russo: "Proinflammatory-activated glioma cells induce a switch in microglial polarization and activation status, from a predominant M2b phenotype to a mixture of M1 and M2a/B polarized cells." in: ASN neuro, Vol. 6, Issue 3, pp. 171-83, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lisi, Laudati, Navarra, Dello Russo: "The mTOR kinase inhibitors polarize glioma-activated microglia to express a M1 phenotype." in: Journal of neuroinflammation, Vol. 11, pp. 125, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lisi, Navarra, Feinstein, Dello Russo: "The mTOR kinase inhibitor rapamycin decreases iNOS mRNA stability in astrocytes." in: Journal of neuroinflammation, Vol. 8, Issue 1, pp. 1, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gupta, Dillon, Ziesmer, Feldman, Witzig, Ansell, Cerhan, Novak: "A proliferation-inducing ligand mediates follicular lymphoma B-cell proliferation and cyclin D1 expression through phosphatidylinositol 3-kinase-regulated mammalian target of rapamycin activation." in: Blood, Vol. 113, Issue 21, pp. 5206-16, 2009

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN153493) back to top
Supplier Images
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (Image 2)
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) Western Blot: TOR/mTOR [p Ser2448] Antibody [ABIN153493] - Detection of a 250 kDa ban...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN153493) Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [ABIN153493] - Used at 5 ug/ml to...