anti-Rat (Rattus) MTOR antibody for Western Blotting

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
C-Term
66
30
28
23
21
18
17
15
15
12
8
7
7
6
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
408
213
205
16
8
6
6
6
6
5
4
3
1
1
1
Host
Rabbit
355
49
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
10
10
9
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
291
157
124
74
57
52
37
17
16
8
7
7
3
3
2
2
2
1
Options
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Catalog No. ABIN2855088
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.92101 ABIN153493 ELISA EM IHC IHC (p) WB Rabbit IgG pSer2448 Log in to see Polyclonal 5
2.92101 ABIN1532744 ELISA WB Rabbit IgG AA 2412-2461 Log in to see Polyclonal 3
2.92101 ABIN1531715 ELISA WB Rabbit IgG AA 2412-2461, pThr2446 Log in to see Polyclonal 3
2.92101 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
2.92101 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal 0
2.92101 ABIN1531910 IHC ELISA WB Rabbit IgG AA 2415-2464, pSer2448 Log in to see Polyclonal 1
2.92101 ABIN1532464 IHC ELISA WB Rabbit IgG AA 2447-2496 Log in to see Polyclonal 0
2.92101 ABIN1532942 IHC ELISA WB Rabbit IgG AA 2415-2464 Log in to see Polyclonal 0
2.92101 ABIN2855089 WB Rabbit IgG Internal Region Log in to see Polyclonal 0
2.92101 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
2.92101 ABIN1842801 IHC WB Rabbit pSer2448 Log in to see Polyclonal 0
2.92101 ABIN1842799 WB Rabbit pThr2446 Log in to see Polyclonal 0
2.92101 ABIN1842805 IF/ICC IHC WB Rabbit pSer2481 Log in to see Polyclonal 0
2.92101 ABIN498698 IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal 0
2.92101 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal 0
2.92101 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal 0
2.92101 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal 0
2.92101 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal 0
2.92101 ABIN3182280 ELISA WB Rabbit IgG pThr2446 Log in to see Polyclonal 0
2.92101 ABIN2737201 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope C-Term
(66), (30), (28), (23), (21), (18), (17), (15), (15), (12), (8), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(408), (213), (205), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1)
Host Rabbit
(355), (49), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(10), (10), (9), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(291), (157), (124), (74), (57), (52), (37), (17), (16), (8), (7), (7), (3), (3), (2), (2), (2), (1)
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Molecular Weight 289 kDa
Gene ID 2475
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunoflu...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse ...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immun...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue ex...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ChIP Image Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of ei...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Various whole c...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse tes...