anti-Rat (Rattus) MTOR antibody for Western Blotting

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pSer2448
66
31
29
22
21
18
16
15
15
11
7
7
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Fish, Human, Mouse (Murine), Rat (Rattus)
406
220
211
16
8
6
6
6
6
5
4
3
1
1
1
1
Host
Rabbit
354
49
3
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
10
10
9
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
294
155
127
69
59
52
33
19
16
8
7
7
3
3
2
2
2
1
1
Supplier
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Catalog No. ABIN6255124
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.064403 ABIN6263424 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 7
3.064403 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
3.064403 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.064403 ABIN153493 ELISA EM IHC IHC (p) WB Rabbit IgG pSer2448 Log in to see Polyclonal 5
3.064403 ABIN1532744 ELISA WB Rabbit IgG AA 2412-2461 Log in to see Polyclonal 3
3.064403 ABIN1531715 ELISA WB Rabbit IgG AA 2412-2461, pThr2446 Log in to see Polyclonal 3
3.064403 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal 0
3.064403 ABIN6256522 ELISA WB Rabbit IgG pThr2446 Log in to see Polyclonal 2
3.064403 ABIN1531910 IHC ELISA WB Rabbit IgG AA 2415-2464, pSer2448 Log in to see Polyclonal 1
3.064403 ABIN1532464 IHC ELISA WB Rabbit IgG AA 2447-2496 Log in to see Polyclonal 0
3.064403 ABIN1532942 IHC ELISA WB Rabbit IgG AA 2415-2464 Log in to see Polyclonal 0
3.064403 ABIN2855089 WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.064403 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal 0
3.064403 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal 0
3.064403 ABIN3182280 ELISA WB Rabbit IgG pThr2446 Log in to see Polyclonal 0
3.064403 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal 0
3.064403 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal 0
3.064403 ABIN6256211 ELISA ICC IF IHC WB Rabbit IgG pSer2481 Log in to see Polyclonal 1
3.064403 ABIN1842801 IHC WB Rabbit pSer2448 Log in to see Polyclonal 0
3.064403 ABIN1842799 WB Rabbit pThr2446 Log in to see Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pSer2448
(66), (31), (29), (22), (21), (18), (16), (15), (15), (11), (7), (7), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Fish, Human, Mouse (Murine), Rat (Rattus)
(406), (220), (211), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1), (1)
Host Rabbit
(354), (49), (3)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(10), (10), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(294), (155), (127), (69), (59), (52), (33), (19), (16), (8), (7), (7), (3), (3), (2), (2), (2), (1), (1)
Pubmed 12 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN6255124) Images
Specificity Phospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Serine 2448
Cross-Reactivity Fish, Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human mTOR around the phosphorylation site of Serine 2448
Isotype IgG
Plasmids, Primers & others

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN6255124) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background

Description: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).

Gene: MTOR

Molecular Weight 289kDa
Gene ID 2475
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN6255124) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN6255124) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-MTOR antibody (ABIN6255124)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Song, Zhang, Sun, Li, Chen, Xiao, Xing: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, 2018

Xue, Ding, Ji, Yan, Huang, Guo, Yang, Sun: "PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats." in: Journal of pharmacological sciences, Vol. 135, Issue 3, pp. 105-113, 2018

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, 2018

Huang, Chen, Zhang, Li, Chen, Yang, Liang, Zhu, Xu: "Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation." in: Journal of molecular neuroscience : MN, Vol. 64, Issue 1, pp. 129-139, 2018

Li, Li, Zeng, Yang, Liu, Zhang, Song, Yao, Ma, Li, Wang, Wei: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, 2018

Wu, Zhu, Yin, Kang, Cao, Tian, Zhang, Lu, Liu: "Traditional Chinese Medicine CFF-1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR-related pathways in prostate cancer." in: Cancer medicine, Vol. 7, Issue 4, pp. 1546-1559, 2018

Zheng, Feng, Jiang, Wu, Tang, Kuang, Zeng, Zhou, Liu: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, 2018

Wu, Zhu, Zhang, Yin, Kang, Cao, Tian, Lu, Liu: "EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7875-7885, 2018

Jin, Hu, Ye, Wu, He: "Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 48, Issue 2, pp. 765-772, 2018

Zhao, Deng, Xu, Zhang, Mi, Meng, Gou, Xu: "PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury." in: Cellular and molecular neurobiology, Vol. 37, Issue 4, pp. 707-715, 2017

He, Cao, Guo, Li, Shang, Liu, Xie, Xu, Liu: "Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia." in: Free radical biology & medicine, Vol. 103, pp. 165-176, 2017

Liu, Luo, Mu, Liu, Geng, Liu, Yi: "Fluoxetine regulates mTOR signalling in a region-dependent manner in depression-like mice." in: Scientific reports, Vol. 5, pp. 16024, 2016

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN6255124) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/100 staining human prostate cancer tissues sections by IHC-P. The ti...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue w...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Western blot analysis of mTOR phosphorylation expression in EGF treated HeLa whole ce...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Phospho-mTOR (Ser2448) Antibody for IHC in human prostate tissue
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Western blot analysis of mTOR phosphorylation expression in mouse liver tissue lysate...