anti-Mouse (Murine) BLK antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BLK Antibody

Antigen
B Lymphoid Tyrosine Kinase (BLK) Antibodies
  • bltk
  • si:dkey-33i22.2
  • zgc:136231
  • MODY11
  • BLK proto-oncogene, Src family tyrosine kinase
  • B lymphoid kinase
  • blk
  • BLK
  • Blk
Epitope
N-Term
22
16
12
10
9
4
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
170
78
51
3
2
1
1
1
1
1
1
Host
Rabbit
113
47
16
Clonality
Polyclonal
Conjugate
This BLK antibody is un-conjugated
6
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
123
104
74
21
13
9
6
5
3
3
2
1
Options
Catalog No. ABIN5552254
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN2883526 ELISA IHC IHC (p) Rabbit IgG pTyr501 Polyclonal 0
1 ABIN719360 IHC (p) WB HRP Rabbit IgG Polyclonal 0
1 ABIN1841212 IHC IHC (p) WB Rabbit AA 1-30 Polyclonal 0
1 ABIN5955886 ELISA IHC (p) WB Rabbit IgG Tyr501 Polyclonal 0
1 ABIN719353 IHC (p) WB Biotin Rabbit IgG Polyclonal 0
1 ABIN719351 IF (p) IHC (p) WB Rabbit IgG Polyclonal 0
1 ABIN6653387 IHC (p) Rabbit pTyr501 Polyclonal 0
1 ABIN6653386 IHC (p) Rabbit pTyr501 Polyclonal 0

General

Antigen B Lymphoid Tyrosine Kinase (BLK) Antibodies
Epitope N-Term
(22), (16), (12), (10), (9), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(170), (78), (51), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(113), (47), (16)
Clonality
Polyclonal
Conjugate This BLK antibody is un-conjugated
(6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(123), (104), (74), (21), (13), (9), (6), (5), (3), (3), (2), (1)
Pubmed 1 reference available
Supplier

Product Details anti-BLK Antibody

Target Details BLK Application Details Handling References for anti-BLK antibody (ABIN5552254) Images
Specificity This antibody reacts to BLK.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BLK.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details BLK

Product Details anti-BLK Antibody Application Details Handling References for anti-BLK antibody (ABIN5552254) Images back to top
Antigen
Alternative Name BLK (BLK Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: B lymphocyte kinase, Tyrosine-protein kinase BLK, p55-BLK, p55BLK
Gene ID 640, 9606
UniProt P51451
Pathways Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-BLK Antibody Target Details BLK Handling References for anti-BLK antibody (ABIN5552254) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BLK Antibody Target Details BLK Application Details References for anti-BLK antibody (ABIN5552254) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BLK antibody (ABIN5552254)

Product Details anti-BLK Antibody Target Details BLK Application Details Handling Images back to top
Product cited in:

Borowiec, Liew, Thompson, Boonyasrisawat, Hu, Mlynarski, El Khattabi, Kim, Marselli, Rich, Krolewski, Bonner-Weir, Sharma, Sale, Mychaleckyj, Kulkarni, Doria: "Mutations at the BLK locus linked to maturity onset diabetes of the young and beta-cell dysfunction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 34, pp. 14460-5, 2009