anti-Rat (Rattus) GABBR1 antibody for Immunohistochemistry

Recommended GABBR1 Antibody (supplied by: Log in to see )

Antigen
gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) Antibodies
  • GABABR1
  • GABBR1-3
  • GB1
  • GPRC3A
  • dJ271M21.1.1
  • dJ271M21.1.2
  • gabbr1
  • GABBR1
  • GABA(B)R1
  • GABAB1
  • GABAbR1
  • bM573K1.1
  • gamma-aminobutyric acid type B receptor subunit 1
  • gamma-aminobutyric acid (GABA) B receptor, 1a
  • gamma-aminobutyric acid (GABA) B receptor, 1
  • GABBR1
  • gabbr1a
  • gabbr1
  • Gabbr1
Epitope
N-Term
73
73
25
14
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
178
164
150
18
13
8
7
6
5
4
3
3
3
1
1
Host
Rabbit
112
88
6
4
2
1
Clonality
Polyclonal
Conjugate
This GABBR1 antibody is un-conjugated
6
6
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
173
71
53
31
28
25
23
14
2
2
2
1
Supplier
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Catalog No. ABIN1048636
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.673489 ABIN1048637 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
8.673489 ABIN2434676 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN446710 IHC (p) IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN574068 IHC IHC (p) Goat AA 947-961 Log in to see Polyclonal 0
1 ABIN3184723 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2889200 ELISA IHC IHC (p) WB Rabbit IgG AA 871-920 Log in to see Polyclonal 0
1 ABIN1098201 IHC WB Mouse IgG1 Cytoplasmic, C-Term, AA 873-977 Log in to see S93A-49 0
1 ABIN601370 IHC WB Guinea Pig IgG AA 972-991 Log in to see Polyclonal 0
1 ABIN2891413 IHC IHC (p) WB Rabbit Ile902 Log in to see Polyclonal 0
1 ABIN1830353 IHC WB Sheep IgG AA 17-586 Log in to see Polyclonal 0
1 ABIN2621128 ICC IHC WB Mouse IgG1 AA 861-912 Log in to see S81-2 0
1 ABIN1582195 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2271160 IHC WB Sheep IgG AA 17-586 Log in to see Polyclonal 0
1 ABIN230152 IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6105940 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2271088 IHC Mouse IgG2a AA 17-586 Log in to see 0
1 ABIN5919990 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6041951 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN571891 IHC WB Rabbit N-Term, Extracellular Domain Log in to see Polyclonal 0
1 ABIN2772427 IHC Rabbit IgG Log in to see Polyclonal 0

General

Antigen gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) Antibodies
Epitope N-Term
(73), (73), (25), (14), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(178), (164), (150), (18), (13), (8), (7), (6), (5), (4), (3), (3), (3), (1), (1)
Host Rabbit
(112), (88), (6), (4), (2), (1)
Clonality
Polyclonal
Conjugate This GABBR1 antibody is un-conjugated
(6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(173), (71), (53), (31), (28), (25), (23), (14), (2), (2), (2), (1)
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Product Details anti-GABBR1 Antibody

Target Details GABBR1 Application Details Handling Images
Brand IHC-plus™
Specificity Human GABBR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Rabbit, Pig, Opossum (100%) Horse (87%) Xenopus, Stickleback, Zebrafish (80%).
Purification Immunoaffinity purified
Immunogen Synthetic 15 amino acid peptide from N-terminal extracellular domain of human GABBR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Rabbit, Pig, Opossum (100%), Horse (87%), Xenopus, Stickleback, Zebrafish (80%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details GABBR1

Product Details anti-GABBR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GABABR1 / GABBR1 (GABBR1 Antibody Abstract)
Background Name/Gene ID: GABBR1
Subfamily: GABA(B)
Family: GPCR

Synonyms: GABBR1, DJ271M21.1.1, DJ271M21.1.2, GABA(B) receptor, Gaba-b-r1a, GABA-BR1, Gaba-br1a receptor, GABAB, subunit 1c, GABAB1, GABA-B receptor 1, Gaba-b1, Gababr1 receptor, GPRC3A, Gabab-receptor subunit 1a, GABABR1, GABBR1-3, GBR1, HGB1a, GABA-B-R1, GB1
Gene ID 2550
Pathways Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process

Application Details

Product Details anti-GABBR1 Antibody Target Details GABBR1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (9 - 18 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-GABBR1 Antibody Target Details GABBR1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-GABBR1 Antibody Target Details GABBR1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) (N-Term) antibody (ABIN1048636) Anti-GABBR1 antibody ABIN1048636 IHC staining of human brain, Purkinje neurons. Immu...