Activin AB (ACVAB) (Active) Protein

Details for Product No. ABIN1019702, Supplier: Log in to see
Protein Name
Origin
Human
Source
Tobacco (Nicotiana benthamiana)
Protein Type
Recombinant
Biological Activity
Active
Application
Cell Culture (CC), Western Blotting (WB)
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Sequence BetaA: GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
BetaB: GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG
Specificity Serological Identification: The protein was analysed by Dot-blot with specifics antibodies
Characteristics Molecular Formula: BetaA: C600H911N173O174S13/ BetaB: C615H910N178O177S12
Isoelectric Point: 6.8
Biological Activity: The biological activity of Activin AB is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 < 5 ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Extinction Coefficient: E 0.1 % (1g/L) = 1.56 (A 280 nm)
Endotoxin Level < 0.04 EU/µg protein (LAL method)
Protein Name
Background Synonyms: Activin beta A beta B heterodimer
Activins are homodimers or heterodimers of the various Beta subunit isoforms, belonging to the TGF-beta family. Mature Activin AB has two chains of 116 and 123 amino acids residues (betaA-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins /Activins are proteins that are formed by the dimerization of two subunits, i. e. an alpha with either betaA -inhibin A- or betaB - inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers calleds activins: Activin A (betaAbetaA), Activin B (betaBbetaB) and Activin AB (betaAbetaB). The activin gene family comprises the additional, but poorly characterized members activin betaC, betaD, and betaE. - As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. - The development of assays distinguishing between different forms of activins and inhibins, along with knock-in and knock-out models, have provided evidence that the betaA- and betaB-subunits have independent and separate roles physiologically. Additionally, evaluation of ligand-receptor interactions indicates significant differences in receptor affinity between activin isoforms, as well as between inhibin isoforms.
UniProt P08476, P09529
Comment

Activin AB is a disulphide linked heterodimer of subunits betaA / betaB . BetaA Single chain, containing 116 aa (13.7 kDa) and BetaB single chain, 123 amino residues (14kDa). Recombinant human Activin AB contains a His-tag at the N-terminal end. Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal Free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Lyophilized protein should be reconstituted in water to a concentration of 50 ng/µL. Optimal concentration should be determined for specific application and cell lines. Optimal concentration should be determined for specific application and cell lines.
Buffer Tris HCl 0.05 M buffer pH 7.4
Storage 4 °C
Supplier Images
 image for Activin AB (ACVAB) (Active) protein (ABIN1019702) SDS-PAGE analysis of human recombinant Activin AB. Lane MWM: molecular weight marker ...
 image for Activin AB (ACVAB) (Active) protein (ABIN1019702) Serological identification. Dot-blot analysis of human recombinant Activin AB, Activi...
 image for Activin AB (ACVAB) (Active) protein (ABIN1019702) The biological activity of Activin AB is measured by its ability to inhibit mouse pla...
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