Activin AB Protein (ACVAB)

Catalog No. ABIN1019702

Product Details

Target: Activin AB (ACVAB)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Application: Cell Culture (CC), Western Blotting (WB)

Sequence: BetaA: GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
BetaB: GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG

Specificity: Serological Identification: The protein was analysed by Dot-blot with specifics antibodies

Characteristics: Molecular Formula: BetaA: C600H911N173O174S13/ BetaB: C615H910N178O177S12
Isoelectric Point: 6.8
Biological Activity: The biological activity of Activin AB is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 < 5 ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Extinction Coefficient: E 0.1 % (1g/L) = 1.56 (A 280 nm)

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

Activin AB is a disulphide linked heterodimer of subunits betaA / betaB . BetaA Single chain, containing 116 aa (13.7 kDa) and BetaB single chain, 123 amino residues (14kDa). Recombinant human Activin AB contains a His-tag at the N-terminal end. Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal Free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 50 ng/µL. Optimal concentration should be determined for specific application and cell lines. Optimal concentration should be determined for specific application and cell lines.

Buffer: Tris HCl 0.05 M buffer pH 7.4

Storage: 4 °C

Target Details

Target: Activin AB (ACVAB)

Abstract: ACVAB Products

Background: Synonyms: Activin beta A beta B heterodimer
Activins are homodimers or heterodimers of the various Beta subunit isoforms, belonging to the TGF-beta family. Mature Activin AB has two chains of 116 and 123 amino acids residues (betaA-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins /Activins are proteins that are formed by the dimerization of two subunits, i. e. an alpha with either betaA -inhibin A- or betaB - inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers calleds activins: Activin A (betaAbetaA), Activin B (betaBbetaB) and Activin AB (betaAbetaB). The activin gene family comprises the additional, but poorly characterized members activin betaC, betaD, and betaE. - As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. - The development of assays distinguishing between different forms of activins and inhibins, along with knock-in and knock-out models, have provided evidence that the betaA- and betaB-subunits have independent and separate roles physiologically. Additionally, evaluation of ligand-receptor interactions indicates significant differences in receptor affinity between activin isoforms, as well as between inhibin isoforms.

UniProt: P08476, P09529