ACE2 Protein (AA 18-740) (His tag)

Catalog No. ABIN1095957

Product Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 18-740

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This ACE2 protein is labelled with His tag.

Application: Functional Studies (Func)

Purpose: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 (C-6His)

Sequence: QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYADQS IKVRISLKSA LGDKAYEWND NEMYLFRSSV AYAMRQYFLK VKNQMILFGE EDVRVANLKP RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLGPPNQP PVSVDHHHHH H

Characteristics: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 produced by transfected human cells is a secreted protein with sequence (Gln18-Ser740) of human ACE-2 fused with a polyhistidine tag at the C-terminus

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Specific Activity is > 800 pmol/min/µg as measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 0.1 mM ZnCl2, 10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

References

Wu, Li, Xia, Tian, Kong, Wang, Gu, Zhang, Tu, Xie, Yang, Lu, Jiang, Ying: "Identification of Human Single-Domain Antibodies against SARS-CoV-2." in: Cell host & microbe, (2020) (PubMed).

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ace-2 (ACE2 Products)

Synonyms: ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein

Sub Type: Fusionprotein

Background: Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.
Alternative Names: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-Converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, ACE2

Molecular Weight: 84.63 kDa

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour