BDH2 Protein (AA 1-245) (His tag)
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- Target See all BDH2 Proteins
- BDH2 (3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-245
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BDH2 protein is labelled with His tag.
- Purpose
- Recombinant Human 3-Hydroxybutyrate Dehydrogenase Type 2/BDH2/DHRS6 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAS EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL
- Characteristics
- Recombinant Human 3-Hydroxybutyrate Dehydrogenase Type 2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu245) of Human BDH2 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BDH2 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- BDH2 (3-hydroxybutyrate Dehydrogenase, Type 2 (BDH2))
- Alternative Name
- BDH2 (BDH2 Products)
- Synonyms
- DHRS6 Protein, BDH2 Protein, bdh2 Protein, EFA6R Protein, PRO20933 Protein, SDR15C1 Protein, UCPA-OR Protein, UNQ6308 Protein, 1810026B04Rik Protein, Dhrs6 Protein, zgc:110323 Protein, 3-hydroxybutyrate dehydrogenase, type 2 Protein, 3-hydroxybutyrate dehydrogenase 2 Protein, 3-hydroxybutyrate dehydrogenase 2 L homeolog Protein, BDH2 Protein, bdh2 Protein, Bdh2 Protein, bdh2.L Protein
- Sub Type
- Fusionprotein
- Background
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3-Hydroxybutyrate Dehydrogenase Type 2 belongs to the short-chain dehydrogenases/reductases (SDR) family. 3-Hydroxybutyrate Dehydrogenase Type 2 may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of 3-Hydroxybutyrate Dehydrogenase Type 2 make it suitable to conbert high levels of circulating 3-hydroxybutyrate into acetoacetate.
Alternative Names: 3-Hydroxybutyrate Dehydrogenase Type 2, Dehydrogenase/Reductase SDR Family Member 6, Oxidoreductase UCPA, R-Beta-Hydroxybutyrate Dehydrogenase, BDH2, DHRS6 - Molecular Weight
- 28.9 kDa
- UniProt
- Q9BUT1
- Pathways
- Transition Metal Ion Homeostasis, Monocarboxylic Acid Catabolic Process
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