CD55 Protein (AA 35-353) (His tag)

Catalog No. ABIN1096332

Product Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Protein Type: Recombinant

Protein Characteristics: AA 35-353

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CD55 protein is labelled with His tag.

Purpose: Recombinant Human CD55/DAF (C-6His)

Sequence: DCGLPPDVPN AQPALEGRTS FPEDTVITYK CEESFVKIPG EKDSVICLKG SQWSDIEEFC NRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREIY CPAPPQIDNG IIQGERDHYG YRQSVTYACN KGFTMIGEHS IYCTVNNDEG EWSGPPPECR GKSLTSKVPP TVQKPTTVNV PTTEVSPTSQ KTTTKTTTPN AQATRSTPVS RTTKHFHETT PNKGSGTTSV DHHHHHH

Characteristics: Recombinant Human CD55 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Asp35-Ser353) of Human CD55 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: cd55 (CD55 Products)

Synonyms: CR Protein, CROM Protein, DAF Protein, TC Protein, Daf Protein, Daf-GPI Protein, Daf1 Protein, GPI-DAF Protein, CD55 molecule (Cromer blood group) Protein, CD55 molecule, decay accelerating factor for complement Protein, CD55 Protein, Cd55 Protein

Sub Type: Fusionprotein

Background: CD55 is a member of the RCA (regulators of complement activation) family. RCA proteins is characterized by the presence of four to 30 SCRs (short consensus repeats also called CCPs for control protein modules) in their plasmaexposed regions. CD55 containing four SCR modules is involved in the regulation of the complement cascade. CD55 is known to bind CD97 via the first SCR. It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on nonforeign cells. It is known that viruses and bacteria also utilize multiple SCR sites for infection.
Alternative Names: Complement Decay-Accelerating factor, CD55, CR, DAF

Molecular Weight: 36 kDa

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity