FCGR2B Protein (AA 43-217) (His tag)

Catalog No. ABIN1096630

Product Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Protein Type: Recombinant

Protein Characteristics: AA 43-217

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This FCGR2B protein is labelled with His tag.

Purpose: Recombinant Human Fc γ RIIb/CD32b (C-6His)

Sequence: TPAAPPKAVL KLEPQWINVL QEDSVTLTCR GTHSPESDSI QWFHNGNLIP THTQPSYRFK ANNNDSGEYT CQTGQTSLSD PVHLTVLSEW LVLQTPHLEF QEGETIVLRC HSWKDKPLVK VTFFQNGKSK KFSRSDPNFS IPQANHSHSG DYHCTGNIGY TLYSSKPVTI TVQAPVDHHH HHH

Characteristics: Recombinant Human FcgammaRIIB/FCGR2B is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Thr43-Pro217) of Human FCGR2B fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: cd32b (FCGR2B Products)

Synonyms: CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein

Sub Type: Fusionprotein

Background: FcgammaRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcgammaRIIA, FcgammaRIIB, and FcgammaRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcgammaRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcgammaRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcgammaRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcgammaRIIB on dendritic cells inhibits maturation and blocks cell activation. FcgammaRIIB may also be a target for monoclonal antibody therapy for malignancies. FcgammaRIIB plays an important negative-regulating role through modulating the signals from activation receptors.
Alternative Names: Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b, IgG Fc Receptor II-b, CDw32, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2

Molecular Weight: 20.64kDa

UniProt: P31994

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling