GADD45G Protein (AA 1-159) (His tag)

Catalog No. ABIN1096795

Product Details

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Protein Type: Recombinant

Protein Characteristics: AA 1-159

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GADD45G protein is labelled with His tag.

Purpose: Recombinant Human GADD45γ/GADD45G (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAQRQGCLT AGVYESAKVL NVDPDNVTFC VLAAGEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR LAAIVGAGEE AGAPGDLHCI LISNPNEDAW KDPALEKLSL FCEESRSVND WVPSITLPE

Characteristics: Recombinant Human Growth Arrest and DNA Damage-Inducible Protein GADD45gamma/GADD45G is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Glu159) of Human GADD45G fused with a 6His tag at the N-terminus. and a 6His at the C-terminus

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Alternative Name: GADD45G (GADD45G Products)

Synonyms: zgc:77806 Protein, GADD45G Protein, XGadd45-gamma Protein, cr6 Protein, ddit2 Protein, gadd45gamma Protein, grp17 Protein, xgadd45g Protein, AI327420 Protein, C86281 Protein, CR6 Protein, DDIT2 Protein, OIG37 Protein, GADD45gamma Protein, GRP17 Protein, growth arrest and DNA-damage-inducible, gamma a Protein, growth arrest and DNA damage inducible gamma Protein, growth arrest and DNA-damage-inducible 45 gamma Protein, growth arrest and DNA-damage-inducible, gamma Protein, gadd45ga Protein, GADD45G Protein, gadd45g Protein, Gadd45g Protein

Sub Type: Fusionprotein

Background: Growth Arrest and DNA Damage-Inducible Protein GADD45 Ypsilon (GADD45G) is a nuclear protein which belongs to the GADD45 family. GADD45G is highly expressed in placenta. GADD45G interacts with various proteins whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45G responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45G is also involved in the regulation of growth and apoptosis. GADD45G inhibits cell growth and differentiation by androgens. The mRNA expression is down-regulated in hepatocellular carcinoma.
Alternative Names: Growth Arrest and DNA Damage-Inducible Protein GADD45 Gamma, Cytokine-Responsive Protein CR6, DNA Damage-Inducible Transcript 2 Protein, DDIT-2, GADD45G, CR6, DDIT2

Molecular Weight: 19.28kDa

UniProt: O95257

Pathways: Cell Division Cycle