NIP7 Protein (AA 1-180) (His tag)

Catalog No. ABIN1097026

Product Details

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Protein Type: Recombinant

Protein Characteristics: AA 1-180

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NIP7 protein is labelled with His tag.

Purpose: Recombinant Human NIP7/KD93 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCF GKFTKTHKFR LHVTALDYLA PYAKYKVWIK PGAEQSFLYG NHVLKSGLGR ITENTSQYQG VVVYSMADIP LGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT

Characteristics: Recombinant Human 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog/NIP7 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr180) of Human NIP7 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0 .

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Alternative Name: KD93 (NIP7 Products)

Synonyms: HSPC031 Protein, KD93 Protein, CGI-37 Protein, Nip7p Protein, pEachy Protein, zgc:110384 Protein, nip7 Protein, Nip7 Protein, ACYPI003208 Protein, NIP7 Protein, DKFZp468F182 Protein, 1110017C15Rik Protein, 6330509M23Rik Protein, AA408773 Protein, AA410017 Protein, PEachy Protein, NIP7, nucleolar pre-rRNA processing protein Protein, NIP7, nucleolar pre-rRNA processing protein L homeolog Protein, 60S ribosome subunit biogenesis protein NIP7 homolog Protein, NIP7 Protein, Nip7 Protein, nip7.L Protein, nip7 Protein

Sub Type: Fusionprotein

Background: 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog (NIP7) belongs to the NIP7 family. NIP7 contains one PUA domain, it is essential for the process of proper 27S pre-rRNA and 60S ribosome subunit assembly. NIP7 is a monomer form and interacts with NOL8 and SBDS, and may bind to RNA. In addition, NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre-ribosomal particles and dissociates as they complete maturation and are exported to the cytoplasm.
Alternative Names: 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog, KD93, NIP7

Molecular Weight: 22.6 kDa

UniProt: Q9Y221

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly