Protein phosphatase 1 (AA 1-171), (Regulatory Subunit 1A) protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- Protein phosphatase 1
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-171, Regulatory Subunit 1A
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A (C-6His)
- Sequence
- MEQDNSPQKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH
- Characteristics
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 1A/PPP1R1A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val171) of Human PPP1R1A.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Protein phosphatase 1 (Regulatory Subunit 15A) protein
Origin: Rat Host: Escherichia coli (E. coli) Recombinant SDS, WB, ELISA, IP
Protein phosphatase 1 (Regulatory Subunit 15A) proteinOrigin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, WB, ELISA, IP
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, 50 % Glycerol, pH 8.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Protein phosphatase 1
- Synonyms
- Ppp1cb Protein, protein phosphatase 1 catalytic subunit beta Protein, PPP1CB Protein
- Background
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Protein Phosphatase 1 Regulatory Subunit 1A (PPP1R1A) is an inhibitor of protein-phosphatase 1. PPP1R1A is a cellular regulator of eIF2 alpha phosphorylation. In hormonal control of glycogen metabolism, IPP-1 protein plays important function. Hormones can elevate intracellular cAMP level and elevate IPP-1 activity. PPP1R1A activation caused cAMP increase , cAMP control over proteins that are not directly phosphorylated by PKA following a rise in intracellular calcium. IPP-1 is inactivated by calcineurin (PP2B). Multiple domains in IPP-1 target cellular PP1 complexes.
Alternative Names: Protein Phosphatase 1 Regulatory Subunit 1A, Protein Phosphatase Inhibitor 1, I-1, IPP-1, PPP1R1A, IPP1 - Molecular Weight
- 19.98 kDa
- UniProt
- Q13522
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