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SEMA5A Protein (AA 23-765) (His tag)

SEMA5A Origin: Human Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1097496
  • Target See all SEMA5A Proteins
    SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 23-765
    Origin
    • 6
    • 1
    Human
    Source
    • 4
    • 1
    • 1
    • 1
    Human Cells
    Purification tag / Conjugate
    This SEMA5A protein is labelled with His tag.
    Purpose
    Recombinant Human Semaphorin-5A/SEMA5A (C-6His)
    Sequence
    EAQGTTQCQR TEHPVISYKE IGPWLREFRA KNAVDFSQLT FDPGQKELVV GARNYLFRLQ LEDLSLIQAV EWECDEATKK ACYSKGKSKE ECQNYIRVLL VGGDRLFTCG TNAFTPVCTN RSLSNLTEIH DQISGMARCP YSPQHNSTAL LTAGGELYAA TAMDFPGRDP AIYRSLGILP PLRTAQYNSK WLNEPNFVSS YDIGNFTYFF FRENAVEHDC GKTVFSRAAR VCKNDIGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ STFFLPELDL IYGIFTTNVN SIAASAVCVF NLSAIAQAFS GPFKYQENSR SAWLPYPNPN PHFQCGTVDQ GLYVNLTERN LQDAQKFILM HEVVQPVTTV PSFMEDNSRF SHVAVDVVQG REALVHIIYL ATDYGTIKKV RVPLNQTSSS CLLEEIELFP ERRREPIRSL QILHSQSVLF VGLREHVVKI PLKRCQFYRT RSTCIGAQDP YCGWDVVMKK CTSLEESLSM TQWEQSISAC PTRNLTVDGH FGVWSPWTPC THTDGSAVGS CLCRTRSCDS PAPQCGGWQC EGPGMEIANC SRNGGWTPWT SWSPCSTTCG IGFQVRQRSC SNPTPRHGGR VCVGQNREER YCNEHLLCPP HMFWTGWGPW ERCTAQCGGG IQARRRICEN GPDCAGCNVE YQSCNTNPCP ELKKTTPWTP WTPVNISDNG GHYEQRFRYT CKARLADPNL LEVGRQRIEM RYCSSDGTSG CSTLDHHHHH H
    Characteristics
    Recombinant Human Semaphorin-5A/SEMA5A produced by transfected human cells is a secreted protein with sequence (Glu23-Thr765) of Human SEMA5A fused with a polyhistidine tag at the C-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product SEMA5A Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 0.1 mM EDTA, 0.05 % Tween 20, pH 7.2.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    4 °C/-20 °C/-80 °C
    Storage Comment
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Expiry Date
    3 months
  • Target
    SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
    Alternative Name
    semaphorin-5a (SEMA5A Products)
    Synonyms
    wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein
    Sub Type
    Fusionprotein
    Background
    Semaphorin-5A (SEMA5A) is a member of the Semaphorin family of axon guidance molecules. SEMA5A is a 140 kDa protein. Class 5 Semaphorins are type I transmembrane glycoproteins with an N- terminal Sema domain and multiple juxtamembrane type 1 Thrombospondin (TSP) repeats within their extracellular domains. SEMA5A is expressed in neuroepithelial cells surrounding retinal axons, oligodendrocytes, the base of limb buds, the mesoderm surrounding cranial vessels , and the cardiac atrial septum and endocardial cushions, Human SEMA5A cDNA encodes a signal sequence, a extracellular domain (ECD), a transmembrane sequence and an cytoplasmic portion. SEMA5A mutations have been implicated in the genetic syndrome,cri-du-chat,while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson. The expression of SEMA5A may be upregulated in metastatic cancer cells and downregulated in autism.
    Alternative Names: Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
    Molecular Weight
    84.71 kDa
    UniProt
    Q13591
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