SUMO2 Protein (AA 1-93) (His tag)

Catalog No. ABIN1097603

Product Details

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Protein Type: Recombinant

Protein Characteristics: AA 1-93

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SUMO2 protein is labelled with His tag.

Purpose: Recombinant Human Small Ubiquitin-Related Modifier 2/SUMO2 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MADEKPKEGV KTENNNHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGGVY

Characteristics: Recombinant Human Small Ubiquitin-Related Modifier 2/SUMO2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gly93) of Human SUMO2.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: sumo2 (SUMO2 Products)

Synonyms: HSMT3 Protein, SMT3B Protein, SMT3H2 Protein, SUMO3 Protein, Smt3A Protein, SMT3A Protein, SMT3H1 Protein, SUMO-3 Protein, Smt3B Protein, Smt3h2 Protein, sumo2 Protein, zgc:92241 Protein, SUMO2 Protein, SUMO-2 Protein, Smt3b Protein, hsmt3 Protein, smt3b Protein, smt3h2 Protein, sumo-2 Protein, PSMT3 Protein, ATSUMO2 Protein, MCO15.11 Protein, MCO15_11 Protein, SMALL UBIQUITIN-LIKE MODIFIER 2 Protein, SUM2 Protein, SUMO 2 Protein, small ubiquitin-like modifier 2 Protein, small ubiquitin-like modifier 2 Protein, small ubiquitin-like modifier 3 Protein, small ubiquitin-like modifier 2b Protein, small ubiquitin-related modifier 2 Protein, small ubiquitin-like modifier 2 S homeolog Protein, small ubiquitin-like modifier 2 L homeolog Protein, SUMO2 Protein, SUMO3 Protein, Sumo2 Protein, sumo2b Protein, LOC714892 Protein, sumo2.S Protein, sumo2.L Protein

Background: Small Ubiquitin-Related Modifier 2 (SUMO2) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
Alternative Names: Small Ubiquitin-Related Modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-Like Protein SMT3A, Smt3A, SUMO2, SMT3A, SMT3H2

Molecular Weight: 13 kDa

UniProt: P61956

Pathways: Methionine Biosynthetic Process